Rabbit Transcription factor jun-B Antibody | anti-JUNB antibody

Anti-JunB Antibody

Cat. Num. AAA177441

• Gene Names: JUNB; AP-1
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: Transcription factor jun-B; Anti-JunB Antibody; Activator protein 1 antibody; AP 1 antibody; AP1 antibody; Jun B antibody; Jun B proto oncogene antibody; Jun B protooncogene antibody; Junb antibody; JunB proto oncogene antibody; JunB protoncogene 9 antibody; JunB protooncogene antibody; JUNB_HUMAN antibody; Transcription factor jun B antibody; Transcription factor jun-B antibody; Transcription factor junB antibody; jun B proto-oncogene; anti-JUNB antibody

• Host: Rabbit
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

• Applicable Applications for anti-JUNB antibody: Western Blot (WB), Immunohistochemistry-Paraffin-embedded Section (IHC-P)
• Application Notes: WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat; IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse; Rat; Antigen Retrieval: By heat; WB: The detection limit for JunB is approximately 0.25ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, ph6.0, for 20mins is required for the staining of formalin/paraffin sections; Other applications have not been tested.; Optimal dilutions should be determined by end users.; ; Antibody can be supported by Chemiluminescent Kit MBS176460 in WB, supported by MBS176451 i n(IHC-P).
• Species: Reacts with: human, mouse, rat
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human JunB (323-347aa QLKQKVMTHVSNGCQLLLGVKGHAF), identical to the related mouse and rat sequences.
• Reconstitution: 0.2ml of distilled water yield a concentration of 500ug/ml
• Preparation and Storage: Store at -20°C. After After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-JunB antibody, MBS177441, Western blottingAll lanes: Anti JunB (MBS177441) at 0.5ug/mlWB: Recombinant Human JunB Protein 0.5ngPredicted bind size: 19KDObserved bind size: 19KD)

Western Blot (WB)

(Anti-JunB antibody, MBS177441, Western blottingAll lanes: Anti JunB (MBS177441) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 36KDObserved bind size: 36KD )

Immunohistochemistry (IHC)

(Anti-JunB antibody, MBS177441, IHC(P)IHC(P): Mouse Brain Tissue )

Immunohistochemistry (IHC)

(Anti-JunB antibody, MBS177441, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-JunB antibody, MBS177441, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-JUNB antibody

Transcription factor jun-B (JUNB) is a protein that in humans is encoded by the JUNB gene. It is mapped to 19p13.2. JUNB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3', and a large fraction (over 50%) of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper transcriptional regulation of this gene. What's more, the expression of JUNB gene might be involved in terminal granulocyte differentiation or in regulating granulocyte functionality.

NCBI and Uniprot Product Information

• NCBI GI #: 4504809
• NCBI GeneID: 3726
• NCBI Accession #: NP_002220.1
• NCBI GenBank Nucleotide #: NM_002229.2
• UniProt Accession #: P17275; Q96GH3
• NCBI Official Full Name: transcription factor jun-B
• NCBI Official Synonym Full Names: jun B proto-oncogene
• NCBI Official Symbol: JUNB
• NCBI Official Synonym Symbols: AP-1
• NCBI Protein Information: transcription factor jun-B; activator protein 1
• UniProt Protein Name: Transcription factor jun-B
• Protein Family: Transcription factor
• UniProt Gene Name: JUNB
• UniProt Entry Name: JUNB_HUMAN

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activity

Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; response to peptide hormone stimulus; response to light stimulus; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; osteoclast differentiation; decidualization; trophectodermal cell differentiation; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis; response to progesterone stimulus; positive regulation of cell differentiation; response to corticosterone stimulus