Rabbit anti-Human RPIA Polyclonal Antibody | anti-RPIA antibody

RPIA, ID (RPIA, RPI, Ribose-5-phosphate isomerase, Phosphoriboisomerase) (AP)

Cat. Num. AAA6343292

• Gene Names: RPIA; RPI; RPIAD
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPIA; Polyclonal Antibody; ID (RPIA; RPI; Ribose-5-phosphate isomerase; Phosphoriboisomerase) (AP); Phosphoriboisomerase; anti-RPIA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RPIA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-207 amino acids from the Central region of human RPIA.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPIA Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the RPIA antibody detected the RPIA protein (arrow).)

Related Product Information for anti-RPIA antibody

The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18.

Product Categories/Family for anti-RPIA antibody

Antibodies; Enzymes; Isomerase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Berry, G.T. J. Inherit. Metab. Dis. 31 (6), 661 (2008): Wamelink, M.M., et al. J. Inherit. Metab. Dis. 31(6):703-717(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 94536842
• NCBI GeneID: 22934
• NCBI Accession #: NP_653164.2
• NCBI GenBank Nucleotide #: NM_144563.2
• UniProt Accession #: P49247; Q541P9; Q96BJ6
• Molecular Weight: 33,269 Da
• NCBI Official Full Name: ribose-5-phosphate isomerase
• NCBI Official Synonym Full Names: ribose 5-phosphate isomerase A
• NCBI Official Symbol: RPIA
• NCBI Official Synonym Symbols: RPI; RPIAD
• NCBI Protein Information: ribose-5-phosphate isomerase; phosphoriboisomerase; ribose 5-phosphate epimerase
• UniProt Protein Name: Ribose-5-phosphate isomerase
• Protein Family: Ribose-5-phosphate isomerase
• UniProt Gene Name: RPIA
• UniProt Synonym Gene Names: RPI
• UniProt Entry Name: RPIA_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18. [provided by RefSeq, Mar 2010]

Uniprot Description

RPIA: Defects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID). A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts. Belongs to the ribose 5-phosphate isomerase family.

Protein type: EC 5.3.1.6; Carbohydrate Metabolism - pentose phosphate pathway; Isomerase

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: intracellular membrane-bound organelle; cytosol

Molecular Function: monosaccharide binding; ribose-5-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; pentose-phosphate shunt, non-oxidative branch; pathogenesis

Disease: Ribose 5-phosphate Isomerase Deficiency