Typical Testing Data/Standard Curve (for reference only)
PAH (p-Aminohippuric Acid) Assay Kit
PAH (p-Aminohippuric Acid) Colorimetric Assay Kit
Synonyms
PAH (p-Aminohippuric Acid); PAH (p-Aminohippuric Acid) Colorimetric Assay Kit; PAH (p-Aminohippuric Acid) assay kit
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PAH (p-Aminohippuric Acid) assay kit
Product Information: PAH (p-Aminohippuric Acid) Assay Kit: Colorimetric Assay to Quantitate PAH in Urine, Plasma etc. within 40 min. Simple & Direct. 100 Assays.
Background: p-Aminohippuric acid (PAH) is a derivative of hippuric acid and useful as a agent for the measurement of renal plasma flow. About 20-30% is completely filtered by the glomerulus and not readsorbed by the tubules. The remainder which bypasses the glomerulus and enters the tubules is completely secreted. At low doses, PAH is almost completely removed with one-pass through the kidneys. Hence, the venous concentration of PAH is close to zero and PAH has been used to determine the effective renal plasma flow (eRPF) from the plasma. The venous concentration is usually <10% that of the plasma concentration so eRPF slightly underestimates the actual RPF. This error is generally accepted because of the ease with which PAH infusion allows calculation of eRPF. In the Assay, PAH reacts with Dimethylaminocinnamaldehyde (DACA) which gives a strongly colored derivative at 550 nm. The amounts of PAH in urine and plasma can easily be quantified.
Summary: Detection method- Absorbance (550 nm)
Species reactivity- Mammalian
Background: p-Aminohippuric acid (PAH) is a derivative of hippuric acid and useful as a agent for the measurement of renal plasma flow. About 20-30% is completely filtered by the glomerulus and not readsorbed by the tubules. The remainder which bypasses the glomerulus and enters the tubules is completely secreted. At low doses, PAH is almost completely removed with one-pass through the kidneys. Hence, the venous concentration of PAH is close to zero and PAH has been used to determine the effective renal plasma flow (eRPF) from the plasma. The venous concentration is usually <10% that of the plasma concentration so eRPF slightly underestimates the actual RPF. This error is generally accepted because of the ease with which PAH infusion allows calculation of eRPF. In the Assay, PAH reacts with Dimethylaminocinnamaldehyde (DACA) which gives a strongly colored derivative at 550 nm. The amounts of PAH in urine and plasma can easily be quantified.
Summary: Detection method- Absorbance (550 nm)
Species reactivity- Mammalian
Product Categories/Family for PAH (p-Aminohippuric Acid) assay kit
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Product Notes
The PAH (p-Aminohippuric Acid) (Catalog #AAA841645) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PAH (p-Aminohippuric Acid), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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