Western Blot (WB)
(Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~87kDa MARCKS protein phosphorylated at Ser152/156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-MARCKS (Ser152 Ser156) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Rabbit MARCKS Polyclonal Antibody | anti-phospho-MARCKS antibody
Anti-phospho-MARCKS (Ser152 Ser156)
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
MARCKS; Polyclonal Antibody; Anti-phospho-MARCKS (Ser152 Ser156); anti-phospho-MARCKS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the MARKS protein phosphorylated at serine 152 and serine 156
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-MARCKS antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~87kDa MARCKS protein phosphorylated at Ser152/156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-MARCKS (Ser152 Ser156) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Western Blot (WB)
(Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~87kDa MARCKS protein phosphorylated at Ser152/156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-MARCKS (Ser152 Ser156) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Related Product Information for anti-phospho-MARCKS antibody
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a member of a family of calmodulin binding proteins thatis a target for phosphorylation by protein kinase C (PKC)(1). MARCKS is concentrated in the synapses of neurons where it appears to function in synaptic vesicle cycling. MARCKS has been shown to bind both actin and calmodulin in vitro (2). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (3). Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (4).
Product Categories/Family for anti-phospho-MARCKS antibody
References
1. Ouimet, C C. et al: Localization of the MARCKS (87kDa) protein, a major specific substrate for protein kinase C, in rat brain. 1990 J Neurosci 10:1683-1698.
2. Hartwig J H. et al: MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 1993356: 618-622.
3. Stumpo D J. et al: MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 1995 92; 944-948.
4. Verghese G M. et al: Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 1994 269:9361-9367.
2. Hartwig J H. et al: MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 1993356: 618-622.
3. Stumpo D J. et al: MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 1995 92; 944-948.
4. Verghese G M. et al: Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 1994 269:9361-9367.
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Product Notes
The phospho-MARCKS (Catalog #AAA516061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-MARCKS (Ser152 Ser156) reacts with Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's MARCKS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the phospho-MARCKS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCKS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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