Renin Active Protein | REN active protein

Human CellExp Renin, mouse recombinant

Cat. Num. AAA844162

• Gene Names: Ren1; Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
• Purity: >=95% by SDS-PAGE gel
• Synonyms: Renin; Human CellExp Renin; mouse recombinant; CYMP; CYM; RENNIN; Chymosin; REN active protein

• Purity/Purification: >=95% by SDS-PAGE gel
• Form/Format: Lyophilized; Lyophilized from 0.22 ?m filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

• Source: HEK 293 cells (Leu22 -Arg402)
• Endotoxin Level: <1 EU/ug by LAL method
• Reconsitution: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXL™ 520. The specific activity is >25 pmol/min/ug.
• Preparation and Storage: Store at -20°C. After reconstitution, aliquot and store at -20°C and use within 3 months. Avoid repeated freezing and thawing cycles.

Testing Data

Related Product Information for REN active protein

Renin, also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), and plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through beta-1 receptor activation. Renin can bind to ATP6AP2, which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, renin binding results in phosphorylation of serine and tyrosine residues of ATP6AP2. The level of renin mRNA appears to be modulated by the binding of HADHB, HuR and CP1 to a regulatory region in the 3' UTR. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.

Product Categories/Family for REN active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Proteins and Enzymes; Proteins and Enzymes (A-Z) Cytokines; Growth Factors & Hormones; Cytokines & Growth Facto

NCBI and Uniprot Product Information

• NCBI GI #: 13676837
• NCBI GeneID: 19701
• NCBI Accession #: NP_112469.1
• NCBI GenBank Nucleotide #: NM_031192.3
• UniProt Accession #: P06281; P97911; Q543E5; Q62153; Q62154
• Molecular Weight: The protein is fused with 6×his tag at the C-terminus and has a calculated MW of 43.2 kDa expressed. The predicted N-terminus is Leu24. Protein migrates as 45-55 kDa in reduced SDS-PAGE due to glycosylation.
• NCBI Official Full Name: renin-1
• NCBI Official Synonym Full Names: renin 1 structural
• NCBI Official Symbol: Ren1
• NCBI Official Synonym Symbols: Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
• NCBI Protein Information: renin-1
• UniProt Protein Name: Renin-1
• Protein Family: Renin
• UniProt Gene Name: Ren1
• UniProt Synonym Gene Names: Ren; Ren-1
• UniProt Entry Name: RENI1_MOUSE

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.15; Protease; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; intracellular

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; hydrolase activity; endopeptidase activity; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to drug; response to cAMP; establishment of blood-nerve barrier; hormone-mediated signaling; renin-angiotensin regulation of aldosterone production; drinking behavior; cell maturation; proteolysis; angiotensin maturation; response to organic substance; regulation of MAPKKK cascade; regulation of blood pressure; renin-angiotensin regulation of blood volume; kidney development