Rabbit anti-Xenopus Polo-Like Kinase Kinase (Ser482,486,490) Polyclonal Antibody | anti-PLK1 antibody

Anti-Phospho-Ser482,486,490 Polo-Like Kinase Kinase

Cat. Num. AAA502103

• Gene Names: plk1; plk
• Reactivity: Xenopus
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Polo-Like Kinase Kinase (Ser482; 486; 490); Polyclonal Antibody; Anti-Phospho-Ser482; 490 Polo-Like Kinase Kinase; anti-PLK1 antibody

• Host: Rabbit
• Reactivity: Xenopus
• Clonality: Polyclonal
• Specificity: Specific for the ~120k PLKK protein phosphorylated at Ser482,486,490.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 598

• Applicable Applications for anti-PLK1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser482,486,490 of Xenopus polo-like kinase kinase (PLKK).
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH
• Immunogen Species: Xenopus
• Reactivity Assumed Based on 100% Sequence Homology: Rat, mouse human zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with Xenopus tissue. It is anticipated that the antibody will react with human, mouse, rat and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Related Product Information for anti-PLK1 antibody

Affinity purified rabbit polyclonal antibody

References

• Erikson E, Haystead TAJ, Qian, Y-W, Maller JL (2004) A feedback loop in the polo-like kinase activation pathway. J Biol Chem 279:32219-32224.
• Kumagai A, Dunphy WG (1996) Purification and molecular cloning of Plx1, a cdc25-regulatory kinase from Xenopus egg extracts. Science 273:1377-1380.
• Liu J, Lewellyn AL, Chen LG, Maller JL (2004) The polo box is required for multiple functions of Plx1 in mitosis. J Biol Chem 279:21367-21373.
• van Vugt, MATM, van de Weerdt, BCM, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RMF, Medema RH (2004) Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/cdc20 activation and initiation of cytokinesis. J Biol Chem 279:36841-36854.
• Eleanor Erikson, Timothy A. J. Haystead, Yue-Wei Qian, and James L. Maller (2004) A Feedback Loop in the Polo-like Kinase Activation Pathway. J. Biol. Chem., Jul 2004; 279: 32219 - 32224

NCBI and Uniprot Product Information

• NCBI GI #: 148235751
• NCBI GeneID: 380481
• NCBI Accession #: NP_001080788.1
• NCBI GenBank Nucleotide #: NM_001087319.1
• UniProt Accession #: P70032; Q7ZWQ6
• Molecular Weight: 120
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo-like kinase 1
• NCBI Official Symbol: plk1
• NCBI Official Synonym Symbols: plk
• NCBI Protein Information: serine/threonine-protein kinase PLK1; plx1; PLK-1
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: plk1
• UniProt Synonym Gene Names: plx1; PLK-1
• UniProt Entry Name: PLK1_XENLA

Uniprot Description

Function: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates cdc25, pkmyt1/myt1, stag2/sa2, tpx2. Plays multiple essential roles during mitosis. Phosphorylates the N-terminal domain of cdc25, which leads to cyclin b-cdc2 activation and mitotic entry. Also required for organization of bipolar spindles, and for exit from mitosis. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating stag2/sa2. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated by phosphorylation of Thr-201. Ref.4

Subunit structure: Interacts with plk1 and kif2a. Ref.9 Ref.10

Subcellular location: Nucleus. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm › cytoskeleton › spindle. Midbody

By similarity. Note: Localized at centrosomes at prophase, spindles at metaphase, and at the midbody during cytokinesis. Ref.4

Induction: By growth-stimulating agents

By similarity. Ref.4

Domain: The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. plk1 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them

By similarity.

Post-translational modification: Activated by phosphorylation on Thr-201 during M phase. Phosphorylated by stk10, leading to activation during oocyte maturation. Ref.3 Ref.4 Ref.5Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Protein levels are down-regulated by proteasomal degradation in anaphase

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 2 POLO box domains.Contains 1 protein kinase domain.