Western Blot (WB)
(Western blot analysis of extracts of HCT116 cells, using ATP8B3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit anti-Human ATP8B3 Polyclonal Antibody | anti-ATP8B3 antibody
ATP8B3 Rabbit pAb
Gene Names
ATP8B3; ATPIK
Reactivity
Human
Applications
Western Blot
Purity
Affinity purification
Synonyms
ATP8B3; Polyclonal Antibody; ATP8B3 Rabbit pAb; ATPIK; anti-ATP8B3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
VTAARNFGYVFLSRTQDTVTIMELGEERVYQVLAIMDFNSTRKRMSVLVRKPEGAICLYTKGADTVIFERLHRRGAMEFATEEALAAFAQETLRTLCLAYREVAEDIYEDWQQRHQEASLLLQNRAQALQQLLGATAIEDRLQDGVPETIKCLKKSNIKIWVLTGDKQETAVNIGFACELLSENMLILEEKEISRILETYWENSNNLLTRESLSQVKLALVINGDFLDKLLVSLRKEPRALAQNVNMDEAWQELGQSRRDFLYARRLSLLCRRFGLPLAAPPAQDSRARRSSEVLQERAFV
Applicable Applications for anti-ATP8B3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 600-900 of human ATP8B3 (NP_620168.1).
Positive Samples
HCT116
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of HCT116 cells, using ATP8B3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of HCT116 cells, using ATP8B3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Related Product Information for anti-ATP8B3 antibody
Background: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to the other. This gene encodes member 3 of phospholipid-transporting ATPase 8B; other members of this protein family are located on chromosomes 1, 15 and 18. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
146,752 Da
NCBI Official Full Name
phospholipid-transporting ATPase IK isoform 2
NCBI Official Synonym Full Names
ATPase, aminophospholipid transporter, class I, type 8B, member 3
NCBI Official Symbol
ATP8B3
NCBI Official Synonym Symbols
ATPIK
NCBI Protein Information
phospholipid-transporting ATPase IK; ATPase, class I, type 8B, member 3; aminophospholipid translocase ATP8B3; probable phospholipid-transporting ATPase IK; potential phospholipid-transporting ATPase IK
UniProt Protein Name
Phospholipid-transporting ATPase IK
Protein Family
UniProt Gene Name
ATP8B3
UniProt Synonym Gene Names
ATP1K; FOS37502_2
UniProt Entry Name
AT8B3_HUMAN
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Product Notes
The ATP8B3 atp8b3 (Catalog #AAA9143054) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP8B3 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP8B3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ATP8B3 atp8b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VTAARNFGYV FLSRTQDTVT IMELGEERVY QVLAIMDFNS TRKRMSVLVR KPEGAICLYT KGADTVIFER LHRRGAMEFA TEEALAAFAQ ETLRTLCLAY REVAEDIYED WQQRHQEASL LLQNRAQALQ QLLGATAIED RLQDGVPETI KCLKKSNIKI WVLTGDKQET AVNIGFACEL LSENMLILEE KEISRILETY WENSNNLLTR ESLSQVKLAL VINGDFLDKL LVSLRKEPRA LAQNVNMDEA WQELGQSRRD FLYARRLSLL CRRFGLPLAA PPAQDSRARR SSEVLQERAF V. It is sometimes possible for the material contained within the vial of "ATP8B3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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