Rabbit anti-Rat EphrinB (Tyr317) Polyclonal Antibody | anti-EPHB2 antibody

Anti-Phospho-Tyr317 EphrinB

Cat. Num. AAA502032

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: EphrinB (Tyr317); Polyclonal Antibody; Anti-Phospho-Tyr317 EphrinB; anti-EPHB2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~46k EphrinB protein phosphorylated at Tyr317. The immunolabeling of the EphrinB band is blocked by treatment with lambda-phosphatase.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 995

• Applicable Applications for anti-EPHB2 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken EphrinB. Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 conjugated to KLH
• Immunogen Species: Chicken
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, chicken, human, mouse, Xenopus and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, chicken, human, mouse, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-EPHB2 antibody

Affinity purified rabbit polyclonal antibody

References

• Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004).
• Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes, Neuron 39:453-465 (2003).
• Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci. 21:309-345 (1998).
• Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M., Kisanuki, Y.Y., Yanagisawa, M., Gale, N.W. and Suda, T, Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells, Blood 100:1326-1333 (2002).
• Weirong Xing, Jonghyun Kim, Jon Wergedal, Shin-Tai Chen, and Subburaman Mohan (2010) Ephrin B1 Regulates Bone Marrow Stromal Cell Differentiation and Bone Formation by Influencing TAZ Transactivation via Complex Formation with NHERF1. Mol. Cell. Biol., 30: 711 - 721.

NCBI and Uniprot Product Information

• NCBI GI #: 46395489
• NCBI GeneID: 396513
• NCBI Accession #: NP_996834.1
• NCBI GenBank Nucleotide #: NM_206951.3
• UniProt Accession #: P28693
• Molecular Weight: 46
• NCBI Official Full Name: ephrin type-B receptor 2
• NCBI Official Synonym Full Names: EPH receptor B2
• NCBI Official Symbol: EPHB2
• NCBI Protein Information: ephrin type-B receptor 2; EK5; cEK5; EPH-like kinase 5; embryo kinase 5 protein CEK5
• UniProt Protein Name: Ephrin type-B receptor 2
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB2
• UniProt Synonym Gene Names: CEK5; EK5; cEK5
• UniProt Entry Name: EPHB2_CHICK

Uniprot Description

Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Beside axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses

By similarity.

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity. Cell projection › axon

By similarity. Cell projection › dendrite

By similarity.

Tissue specificity: Wide tissue distribution throughout development and sustained expression in adult brain. The longer form (CEK5+) is specifically expressed in the central nervous system.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.

Sequence caution: The sequence AAA48667.1 differs from that shown. Reason: Erroneous initiation.