Typical Testing Data/Standard Curve (for reference only)
Human Glucose Regulated Protein 78 (GRP78) ELISA Kit | GRP78 elisa kit
Human Glucose Regulated Protein 78 (GRP78) ELISA Kit
Reactivity
Human
Synonyms
Glucose Regulated Protein 78 (GRP78); Human Glucose Regulated Protein 78 (GRP78) ELISA Kit; GRP78 elisa kit
Reactivity
Human
Sequence Length
682
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.5-100ng/ml
Sensitivity
0.23ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV< 10%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV< 12%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GRP78 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Glucose Regulated Protein 78 (also known as GRP78) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GRP78 antibody. GRP78 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GRP78 Antibody is added and binds to GRP78 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GRP78 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GRP78. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GRP78 antibody. GRP78 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GRP78 Antibody is added and binds to GRP78 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GRP78 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GRP78. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
74,468 Da
NCBI Official Full Name
glucose regulated protein 78
UniProt Protein Name
Endoplasmic reticulum chaperone BiP
Protein Family
UniProt Gene Name
KAR2
UniProt Synonym Gene Names
GRP781 PublicationManual assertion based on opinion iniRef.1; GRP-781 PublicationManual assertion based on opinion iniRef.1; BiPCurated
Uniprot Description
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.
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Product Notes
The Human GRP78 kar2 (Catalog #AAA166969) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166969 ELISA Kit recognizes Human GRP78. It is sometimes possible for the material contained within the vial of "Glucose Regulated Protein 78 (GRP78), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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