Rabbit anti-Rat MEK5 (Ser311, Thr315) Polyclonal Antibody | anti-MAP2K5 antibody

Anti-Phospho-Ser311, Thr315 MEK5

Cat. Num. AAA502075

• Gene Names: Map2k5; Mek5
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: MEK5 (Ser311; Thr315); Polyclonal Antibody; Anti-Phospho-Ser311; Thr315 MEK5; anti-MAP2K5 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 448

• Applicable Applications for anti-MAP2K5 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, chicken, bovine, canine, non-human primate, Xenopus, and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-MAP2K5 antibody

Affinity purified rabbit polyclonal antibody

References

• Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 270(21):12665-9.
• Chao TH, Hayashi M, Tapping RI, Kato Y, Lee JD (1999) MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway. J. Biol. Chem. 274(51):36035-8.
• Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY (2003) MEK5 overexpression is associated with metastatic prostrate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 22(9):1381-9.
• Cameron SJ, Abe J, Malik S, Che W, Yang J (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation. J. Biol. Chem. 279(2):1506-12.

NCBI and Uniprot Product Information

• NCBI GI #: 77157802
• NCBI GeneID: 29568
• NCBI Accession #: NP_001029159.1
• NCBI GenBank Nucleotide #: NM_001033987.1
• UniProt Accession #: Q62862; Q62863; Q62864
• Molecular Weight: 49
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform a
• NCBI Official Synonym Full Names: mitogen activated protein kinase kinase 5
• NCBI Official Symbol: Map2k5
• NCBI Official Synonym Symbols: Mek5
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; MAP kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k5
• UniProt Synonym Gene Names: Mek5; Mkk5; Prkmk5; MAP kinase kinase 5; MAPKK 5; MEK 5
• UniProt Entry Name: MP2K5_RAT

NCBI Description

kinase involved in signal transduction in the cell [RGD, Feb 2006]

Uniprot Description

Function: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis

By similarity. Ref.1

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI

By similarity.

Subcellular location: Isoform Beta: Cytoplasm › cytosol. Isoform Alpha-1: Membrane. Note: The alternatively spliced exon in alpha isoform resembleconserved sequences that mediate interactions with the cytoskeleton, thereby explaining the differential localization.Isoform Alpha-2: Membrane. Note: The alternatively spliced exon in alpha isoform resembleconserved sequences that mediate interactions with the cytoskeleton, thereby explaining the differential localization.

Tissue specificity: MEK5 beta is ubiquitously distributed with highest levels in the liver, whereas MEK5 alpha is expressed only in liver and brain.

Domain: Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A

By similarity.

Post-translational modification: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 OPR domain.Contains 1 protein kinase domain.