Rabbit anti-Rat Munc-18 (Ser 515) Polyclonal Antibody | anti-STXBP2 antibody

Anti-Phospho-Ser515 Munc-18

Cat. Num. AAA502078

• Gene Names: Stxbp2; Munc18b
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Munc-18 (Ser 515); Polyclonal Antibody; Anti-Phospho-Ser515 Munc-18; anti-STXBP2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 594

• Applicable Applications for anti-STXBP2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:400
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser515 of Munc-18.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser515 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates and Xenopus
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-STXBP2 antibody

Affinity purified rabbit polyclonal antibody

References

• Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
• Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.
• Sassa T, Ogawa H, Kimoto M, Hosono R (1996) The synaptic protein UNC-18 is phosphorylated by protein kinase C. Neurochem Int 29:543-552.
• Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-Lorenzo, Pedro Zamorano, Eckart D. Gundelfinger, and Craig C. Garner (2008) Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 - 846.

NCBI and Uniprot Product Information

• NCBI GI #: 13592101
• NCBI GeneID: 81804
• NCBI Accession #: NP_112388.1
• NCBI GenBank Nucleotide #: NM_031126.1
• UniProt Accession #: Q62753
• Molecular Weight: 65
• NCBI Official Full Name: syntaxin-binding protein 2
• NCBI Official Synonym Full Names: syntaxin binding protein 2
• NCBI Official Symbol: Stxbp2
• NCBI Official Synonym Symbols: Munc18b
• NCBI Protein Information: syntaxin-binding protein 2; unc-18B; unc18-2; munc18-2; unc-18 homolog 2; protein unc-18 homolog 2; protein unc-18 homolog B; syntaxin binding protein Munc18-2
• UniProt Protein Name: Syntaxin-binding protein 2
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: Stxbp2
• UniProt Synonym Gene Names: Unc18b; Munc18-2; Unc18-2; Unc-18B
• UniProt Entry Name: STXB2_RAT

NCBI Description

syntaxin binding protein; important for synaptic vesicle exoctyosis; may be a component of the SNARE complex; may be involved with pancreatic beta-cell exocytosis [RGD, Feb 2006]

Uniprot Description

Function: Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells

By similarity.

Subunit structure: Interacts with STX1A, STX2 and STX3

By similarity. Interacts with STX11

By similarity.

Sequence similarities: Belongs to the STXBP/unc-18/SEC1 family.