Rabbit anti-Human, rat 14-3-3 (Ser58) Polyclonal Antibody | anti-YWHAB antibody

Anti-Phospho-Ser58 14-3-3 Protein

Cat. Num. AAA502001

• Reactivity: Human, rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: 14-3-3 (Ser58); Polyclonal Antibody; Anti-Phospho-Ser58 14-3-3 Protein; anti-YWHAB antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 246

• Applicable Applications for anti-YWHAB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:500
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat 14-3-3 protein.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human and rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen for this antibody.
• Biological Significance: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-YWHAB antibody

Affinity purified rabbit polyclonal antibody

References

• Berg D, Holzmann C, Riess O (2003) 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 4:752-762.
• Bridges D, Moorhead GB (2005) 14-3-3 Proteins: a number of functions for a numbered protein. Sci STKE 2005:re10.
• Dougherty MK, Morrison DK (2004) Unlocking the code of 14-3-3. J Cell Sci 117:1875-1884.
• Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, Obsil T (2004) 14-3-3 Protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding. J Biol Chem 279:49113-49119.
• Woodcock JM, Murphy J, Stomski FC, Berndt MC, Lopez AF (2003) The dimeric versus monomeric status of 14-3-3 zeta is controlled by phosphorylation of Ser58 at the dimer interface. J Biol Chem 278:36323-36327.

NCBI and Uniprot Product Information

• NCBI GI #: 9507243
• NCBI GeneID: 56011
• NCBI Accession #: NP_062250.1
• NCBI GenBank Nucleotide #: NM_019377.1
• UniProt Accession #: P35213
• Molecular Weight: 29
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
• NCBI Official Symbol: Ywhab
• NCBI Protein Information: 14-3-3 protein beta/alpha; KCIP-1; 14-3-3 protein beta-subtype; prepronerve growth factor RNH-1; protein kinase C inhibitor protein 1; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
• UniProt Protein Name: 14-3-3 protein beta/alpha
• Protein Family: 14-3-3 protein
• UniProt Gene Name: Ywhab
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_RAT

NCBI Description

member of the 14-3-3 protein family; may mediate signal transduction by binding to phosphoserine-containing proteins; may play a role in cell cycle regulation [RGD, Feb 2006]

Uniprot Description

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2

By similarity.

Subunit structure: Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1 (phosphorylated form); the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2, YAP1 and PRKCE (phosphorylated form). Interacts with SAMSN1

By similarity. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2

By similarity. Ref.5

Subcellular location: Cytoplasm. Melanosome

By similarity.

Post-translational modification: The alpha, brain-specific form differs from the beta form in being phosphorylated

By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion

By similarity.Isoform Short contains a N-acetylmethionine at position 1

By similarity.

Sequence similarities: Belongs to the 14-3-3 family.