Rabbit anti-Rat Alpha synuclein (ser129) Polyclonal Antibody | anti-SNCA antibody

Anti-Phospho-Ser129 Alpha Synuclein

Cat. Num. AAA502004

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Alpha synuclein (ser129); Polyclonal Antibody; Anti-Phospho-Ser129 Alpha Synuclein; anti-SNCA antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~15 kDa alpha-synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 140

• Applicable Applications for anti-SNCA antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser129 of alpha synuclein.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse, and non-human primate
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson's disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-SNCA antibody

Affinity purified rabbit polyclonal antibody

References

• Okochi M, Walter J, Koyama A, Nakajo S, Baba M, Iwatsubo T, Meijer L, Kahle PJ, Haass C (2000) Constitutive phosphorylation of the Parkinson's disease associated alphasynuclein. J Biol Chem Jan 7;275(1): 390-7.
• Neumann M, Kahle PJ, Giasson BI, Ozmen L, Borroni E, Spooren W, Muller V, Odoy S, Fujiwara H, Hasegawa M, Iwatsubo T, Trojanowski JQ, Kretzschmar HA, Haass C (2002) Misfolded proteinase k resistant hyperphosphorylated alpha-synuclein in aged transgenic mice with locomotor deterioration and in human alpha synucleinopathies. J Clin Invest Nov; 110(10):1403-5.
• Smith WW, Margolis RL, Li X, Troncoso JC, Lee MK, Dawson VL, Dawson TM, Iwatsubo T, Ross CA (2005) Alpha-synuclein phosphorylation enhances eosinophilic cytoplasmic inclusion formation in SH-SY5Y Cells. J Neurosci June 8; 25(23):5544-5552.
• Masliah E, Rockenstein E, Adame A, Alford M, Crews L, Hashimoto M, Suebert P, Lee M, Goldstein J, Chilcote T, Games D, Schenk D (2005) Effects of alpha-synuclein immunization in a mouse model of Parkinson's disease. Neuron Jun 16;46(6):857-868.

NCBI and Uniprot Product Information

• NCBI GI #: 9507125
• NCBI GeneID: 29219
• NCBI Accession #: NP_062042.1
• NCBI GenBank Nucleotide #: NM_019169.2
• UniProt Accession #: P37377; P37378; Q53YM9
• Molecular Weight: 15
• NCBI Official Full Name: alpha-synuclein
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: Snca
• NCBI Protein Information: alpha-synuclein
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: Snca
• UniProt Entry Name: SYUA_RAT

NCBI Description

may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD, Feb 2006]

Uniprot Description

Function: May be involved in the regulation of dopamine release and transport.

Subunit structure: Interacts with UCHL1. Ref.7

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Found only in brain (hippocampus, brainstem and cortex). Specifically expressed in neuronal cell bodies and synapses.

Post-translational modification: Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress

By similarity.Ubiquitinated. The predominant conjugate is the diubiquitinated form. Ref.7Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure

By similarity.

Sequence similarities: Belongs to the synuclein family.