Western Blot (WB)
(Anti-PACSIN3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:54 kDa)
Rabbit anti-Human PACSIN3 Polyclonal Antibody | anti-PACSIN3 antibody
Anti-PACSIN3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PACSIN3; Polyclonal Antibody; Anti-PACSIN3 Antibody; Rabbit Polyclonal; PACSIN3 Antibody; Rabbit PAb; Antigen Affinity Purified; protein kinase C and casein kinase substrate in neurons 3; Anti-SDPIII Antibody; anti-PACSIN3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PACSIN3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PACSIN3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:5000
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human PACSIN3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PACSIN3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PACSIN3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:54 kDa)
Western Blot (WB)
(Anti-PACSIN3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:54 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PACSIN3 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PACSIN3 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(PACSIN3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-PACSIN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PACSIN3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa Observed band size :54 kDa)
Immunoprecipitation (IP)
(PACSIN3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-PACSIN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PACSIN3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa Observed band size :54 kDa)
Related Product Information for anti-PACSIN3 antibody
PACSIN3 (Protein Kinase C And Casein Kinase Substrate In Neurons 3) is a Protein Coding gene. The PACSIN3 gene, located on 11p11.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. Diseases associated with PACSIN3 include Hereditary Motor And Sensory Neuropathy, Type Iic and Metatropic Dysplasia. Among its related pathways are Vesicle-mediated transport and Clathrin-mediated endocytosis. PACSIN3 has been shown to interact with PACSIN1 and PACSIN2.
References
Samasilp P, et al. (2014) Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells. Am J Physiol Cell Physiol 306 (9): C831-843.Kessels MM, et al. (2004) The syndapin protein family: Linking membrane trafficking with the cytoskeleton. J Cell Sci 117 (Pt 15): 3077-3086.Goh SL, et al. (2012) Versatile membrane deformation potential of activated pacsin. PLoS One 7 (12): e51628.
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Product Notes
The PACSIN3 (Catalog #AAA8107996) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PACSIN3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PACSIN3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:1000-1:5000 IHC-P: 1:50-1:200 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PACSIN3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PACSIN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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