Rabbit NDUFS2 Polyclonal Antibody | anti-NDUFS2 antibody

Anti-NDUFS2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109092

• Reactivity: Human, Cynomolgus
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NDUFS2; Polyclonal Antibody; Anti-NDUFS2 Antibody; Rabbit Polyclonal; NDUFS2 Antibody; Rabbit PAb; Antigen Affinity Purified; NADH dehydrogenase (ubiquinone) Fe-S protein 2; 49kDa (NADH-coenzyme Q reductase); Anti-CI-49 Antibody; anti-NDUFS2 antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NDUFS2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NDUFS2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human NDUFS2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NDUFS2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NDUFS2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:50 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human NDUFS2 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(NDUFS2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-NDUFS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFS2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa Observed band size :45 kDa)

Related Product Information for anti-NDUFS2 antibody

NDUFS2 (NADH: Ubiquinone Oxidoreductase Core Subunit S2) is a Protein Coding gene. The NDUFS2 gene, located on 1q23.3, is conserved in chimpanzee, Rhesus monkey, dog, and cow, etc. NDUFS2 belongs to the complex I 49 kDa subunit family. The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). The NDUFS2 protein is part of complex I of many pro-and eukaryotes. The nuclear gene coding for this protein is therefore an important candidate for mutational detection studies in enzymatic complex I deficient patients. Diseases associated with NDUFS2 include Mitochondrial Complex I Deficiency, Nuclear Type 6, and Leigh Syndrome With Cardiomyopathy. Among its related pathways are GABAergic synapse and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.

References

Loeffen J, et al. (2001) Mutations in the complex i ndufs2 gene of patients with cardiomyopathy and encephalomyopathy. Ann Neurol 49 (2): 195-201.Liu L, et al. (2019) S100a4 alters metabolism and promotes invasion of lung cancer cells by up-regulating mitochondrial complex i protein ndufs2. J Biol Chem 294 (18): 7516-7527.Gerber S, et al. (2017) Compound heterozygosity for severe and hypomorphic ndufs2 mutations cause non-syndromic lhon-like optic neuropathy. J Med Genet 54 (5): 346-356.