Western Blot (WB)
(Anti-PSMD5 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: OVCAR3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:55 kDa)
Rabbit anti-Human PSMD5 Polyclonal Antibody | anti-PSMD5 antibody
Anti-PSMD5 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PSMD5; Polyclonal Antibody; Anti-PSMD5 Antibody; Rabbit Polyclonal; PSMD5 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome 26S subunit; non-ATPase 5; Anti-S5B Antibody; anti-PSMD5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMD5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PSMD5 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human PSMD5 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PSMD5 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PSMD5 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: OVCAR3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:55 kDa)
Western Blot (WB)
(Anti-PSMD5 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: OVCAR3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:55 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PSMD5 in human prostate with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PSMD5 in human prostate with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(PSMD5 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-PSMD5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMD5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa Observed band size :55 kDa)
Immunoprecipitation (IP)
(PSMD5 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-PSMD5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMD5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa Observed band size :55 kDa)
Related Product Information for anti-PSMD5 antibody
PSMD5 (Proteasome 26S Subunit, Non-ATPase 5) is a Protein Coding gene. The PSMD5 gene, located on 9q33.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, and frog. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. PSMD5 is widely expressed in the urinary bladder, adrenal, and other tissues. Diseases associated with PSMD5 include Localized Pulmonary Fibrosis and Kaufman Oculocerebrofacial Syndrome. The related pathways of PSMD5 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation. 242 organisms have orthologs with the human gene PSMD5.
References
Kanayama HO, et al. (1992) Demonstration that a human 26s proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes atp and ubiquitin-ligated proteins by closely linked mechanisms. Eur J Biochem 206 (2): 567-578.Deveraux Q, et al. (1995) Molecular cloning and expression of a 26 s protease subunit enriched in dileucine repeats. J Biol Chem 270 (40): 23726-23729.Deveraux Q, et al. (1994) A 26 s protease subunit that binds ubiquitin conjugates. J Biol Chem 269 (10): 7059-7061.
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Product Notes
The PSMD5 (Catalog #AAA8108086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMD5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMD5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PSMD5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMD5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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