Flow Cytometry (FC/FACS)
(Staining of the HeLa cell line with 0.25 ug of Mouse IgG1 K Isotype Control (biotin) (blue histogram) or 0.25 ug of CD325 antibody (N-Cadherin) (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Mouse CD325 Monoclonal Antibody | anti-CD325 antibody
CD325 antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD325; Monoclonal Antibody; CD325 antibody (biotin); Monoclonal CD325; Anti-CD325; CD 325; CD-325; neural cadherin; anti-CD325 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
8C11
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-CD325 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Biological Significance
The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of the HeLa cell line with 0.25 ug of Mouse IgG1 K Isotype Control (biotin) (blue histogram) or 0.25 ug of CD325 antibody (N-Cadherin) (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of the HeLa cell line with 0.25 ug of Mouse IgG1 K Isotype Control (biotin) (blue histogram) or 0.25 ug of CD325 antibody (N-Cadherin) (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-CD325 antibody
Mouse monoclonal CD325 antibody (biotin)
Product Categories/Family for anti-CD325 antibody
Similar Products
Product Notes
The CD325 (Catalog #AAA832686) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD325 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.125 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD325 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD325, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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