Rabbit anti-Human RBM17 Polyclonal Antibody | anti-RBM17 antibody

Anti-RBM17 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108813

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RBM17; Polyclonal Antibody; Anti-RBM17 Antibody; Rabbit Polyclonal; RBM17 Antibody; Rabbit PAb; Antigen Affinity Purified; RNA binding motif protein 17; Anti-SPF45 Antibody; anti-RBM17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RBM17
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RBM17 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human RBM17 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human RBM17 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RBM17 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size:45 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human RBM17 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(RBM17 was immunoprecipitated using: Lane A:0.5 mg Raji Whole Cell Lysate 4 uL anti-RBM17 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBM17 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size :45 kDa)

Related Product Information for anti-RBM17 antibody

RBM17 (RNA Binding Motif Protein 17, also known as SPF45) is a Protein Coding gene. The RBM17 gene, located on 10p15.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, and mouse, etc. This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. RBM17 is frequently overexpressed in many tumors and plays a crucial role in cancer progression and drug resistance. RBM17 may be a therapeutic target in the clinical management of glioma. RBM17 is frequently overexpressed in many solid tumors, and stable expression in HeLa cells confers resistance to doxorubicin and vincristine. Diseases associated with RBM17 include Spinocerebellar Ataxia 1 and Autosomal Dominant Cerebellar Ataxia.

References

Perry WL, 3rd, et al. (2005) Human splicing factor spf45 (rbm17) confers broad multidrug resistance to anticancer drugs when overexpressed--a phenotype partially reversed by selective estrogen receptor modulators. Cancer Res 65 (15): 6593-6600.Lu J, et al. (2018) Rbm17 controls apoptosis and proliferation to promote glioma progression. Biochem Biophys Res Commun 505 (1): 20-28.Li C, et al. (2020) Exploration of the effects of the cyclops gene rbm17 in hepatocellular carcinoma. PLoS One 15 (6): e0234062.