Rabbit anti-Human MSTO1 Polyclonal Antibody | anti-MSTO1 antibody

Anti-MSTO1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108052

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MSTO1; Polyclonal Antibody; Anti-MSTO1 Antibody; Rabbit Polyclonal; MSTO1 Antibody; Rabbit PAb; Antigen Affinity Purified; misato 1; mitochondrial distribution and morphology regulator; Anti-LST005 Antibody; Anti-MST Antibody; anti-MSTO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MSTO1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MSTO1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: E Coli-derived Human MSTO1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MSTO1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MSTO1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa)

Immunoprecipitation (IP)

(MSTO1 was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 1 uL anti-MSTO1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MSTO1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size :55 kDa)

Related Product Information for anti-MSTO1 antibody

MSTO1 (Misato Mitochondrial Distribution And Morphology Regulator 1) is a Protein Coding gene. The MSTO1 gene, located on 1q22, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. MSTO1 belongs to the misato family. 7 alternatively spliced human isoforms have been reported. MSTO1 encodes a cytosolic mitochondrial fusion protein, misato homolog 1 or MSTO1. Human MSTO1 is involved in the regulation of mitochondrial distribution and morphology and its unregulated expression leads to a mitochondrial disorder. MSTO1 mutations have been shown to cause clinical manifestations suggestive of mitochondrial dysfunction, such as muscle weakness, short stature, motor developmental delay, and cerebellar atrophy. Both autosomal dominant and recessive modes of inheritance have been suggested.

References

Li K, et al. (2020) Whole-exome sequencing identifies rare compound heterozygous mutations in the msto1 gene associated with cerebellar ataxia and myopathy. Eur J Med Genet 63 (1): 103623.Iwama K, et al. (2018) Novel recessive mutations in msto1 cause cerebellar atrophy with pigmentary retinopathy. J Hum Genet 63 (3): 263-270.Donkervoort S, et al. (2019) Msto1 mutations cause mtdna depletion, manifesting as muscular dystrophy with cerebellar involvement. Acta Neuropathol 138 (6): 1013-1031.