Rabbit anti-Human PSMC4 Polyclonal Antibody | anti-PSMC4 antibody

Anti-PSMC4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108074

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMC4; Polyclonal Antibody; Anti-PSMC4 Antibody; Rabbit Polyclonal; PSMC4 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) 26S subunit; ATPase; 4; Anti-MIP224 Antibody; Anti-RPT3 Antibody; Anti-S6 Antibody; Anti-TBP-7 Antibody; Anti-TBP7 Antibody; anti-PSMC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMC4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMC4 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human PSMC4 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PSMC4 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMC4 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: U251 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:50 kDa)

Immunoprecipitation (IP)

(PSMC4 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 4 uL anti-PSMC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMC4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size :50 kDa)

Related Product Information for anti-PSMC4 antibody

PSMC4 (Proteasome 26S Subunit, ATPase 4) is a Protein Coding gene. The PSMC4 gene, located on 19q13.11-q13.13, is conserved in chimpanzee, Rhesus monkey, dog, cow, and mouse, etc. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. PSMC3 and PSMC4, components of the 19S complex of the 26S proteasome, show a significant degree of amino acid similarity, especially in the conserved ATPase domain (CAD). Diseases associated with PSMC4 include Episodic Ataxia, Type 2, and Cystic Fibrosis.

References

Zhu Y, et al. (2018) Structural mechanism for nucleotide-driven remodeling of the aaa-atpase unfoldase in the activated human 26s proteasome. Nat Commun 9 (1): 1360.Sakao Y, et al. (2000) Mouse proteasomal atpases psmc3 and psmc4: Genomic organization and gene targeting. Genomics 67 (1): 1-7.Mojic M, et al. (2012) Saquinavir-no-targeted s6 protein mediates sensitivity of androgen-dependent prostate cancer cells to trail. Cell Cycle 11 (6): 1174-1182.