Rabbit anti-Human PDHX Polyclonal Antibody | anti-PDHX antibody

Anti-PDHX Antibody, Rabbit Polyclonal

Cat. Num. AAA8109545

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PDHX; Polyclonal Antibody; Anti-PDHX Antibody; Rabbit Polyclonal; PDHX Antibody; Rabbit PAb; Antigen Affinity Purified; pyruvate dehydrogenase complex; component X; Anti-DLDBP Antibody; Anti-E3BP Antibody; Anti-OPDX Antibody; Anti-PDX1 Antibody; Anti-proX Antibody; anti-PDHX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PDHX
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PDHX antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human PDHX fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PDHX fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PDHX rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: NCI H460 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PDHX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of PDHX in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDHX polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(PDHX was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-PDHX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PDHX rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa Observed band size :55 kDa)

Related Product Information for anti-PDHX antibody

PDHX (Pyruvate Dehydrogenase Complex Component X) is a Protein Coding gene. The PDHX gene, located on 11p13, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, chicken, zebrafish, and frog. This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). Among its related pathways are Pyruvate metabolism and Citric Acid (TCA) cycle and Signaling by GPCR.

Product Categories/Family for anti-PDHX antibody

Adaptor Proteins

References

Ivanov IS, et al. (2014) Founder p.Arg 446* mutation in the pdhx gene explains over half of cases with congenital lactic acidosis in roma children. Mol Genet Metab 113 (1-2): 76-83.Brown RM, et al. (2006) Pyruvate dehydrogenase e3 binding protein (protein x) deficiency. Dev Med Child Neurol 48 (9): 756-760.Bottone MG, et al. (2007) Enzyme-assisted photosensitization with rose bengal acetate induces structural and functional alteration of mitochondria in hela cells. Histochem Cell Biol 127 (3): 263-271.