Goat Phosphoinositide 3 Kinase, p55 gamma Polyclonal Antibody | anti-PIK3R3 antibody

Phosphoinositide 3 Kinase, p55 gamma (PI-3 Kinase p55 gamma, Phosphoinositide-3-kinase Regulatory Subunit 3 (p55, gamma), PIK3R3)

Cat. Num. AAA622372

• Gene Names: PIK3R3; p55; p55-GAMMA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Phosphoinositide 3 Kinase; p55 gamma; Polyclonal Antibody; p55 gamma (PI-3 Kinase p55 gamma; Phosphoinositide-3-kinase Regulatory Subunit 3 (p55; gamma); PIK3R3); Anti -Phosphoinositide 3 Kinase; anti-PIK3R3 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous human PI 3-Kinase p55g at 55-57kD. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-PIK3R3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: E. coli-derived recombinant human PI 3-Kinase p55g (aa251-378).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of PI 3-Kinase p55g with MBS622372. Lysates from human SHSY5Y, mouse C2C12, and rat Rat-2 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-PI 3-Kinase p55g.)

Related Product Information for anti-PIK3R3 antibody

p55g (Protein of 55kD, gamma subunit; also PI 3-Kinase regulatory subunit gamma and p55 PIK) is a 55-57kD member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55g/p55 PIK should not be confused with the 55kD alpha splice variant of the p85 alpha gene (75% aa identity). Human p55g is 461aa in length and contains a unique 34aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa35-44) and two SH2 domains that bind the p110 catalytic subunit (aa65-160 and 358-452).

Product Categories/Family for anti-PIK3R3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 166795247
• NCBI GeneID: 8503
• NCBI Accession #: NP_001107644.1
• NCBI GenBank Nucleotide #: NM_001114172.1
• UniProt Accession #: Q92569; O60482; Q5T4P1; Q5T4P2; B2R9C1; D3DQ12
• Molecular Weight: 54,448 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit gamma
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
• NCBI Official Symbol: PIK3R3
• NCBI Official Synonym Symbols: p55; p55-GAMMA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit gamma; p55PIK; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; 100% homology to SWISS-PROT Q92569; PI3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R3
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma
• UniProt Entry Name: P55G_HUMAN

Uniprot Description

PIK3R3: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Belongs to the PI3K p85 subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity

Biological Process: phospholipid metabolic process; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; regulation of phosphoinositide 3-kinase activity