Mouse Microphthalmia Monoclonal Antibody | anti-Mitf antibody

Microphthalmia (Transcription Factor, MiTF)

Cat. Num. AAA601541

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Microphthalmia; Monoclonal Antibody; Microphthalmia (Transcription Factor; MiTF); Anti -Microphthalmia (Transcription Factor; anti-Mitf antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4H205
• Specificity: Recognizes human Microphthalmia (Transcription Factor, MiTF). Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-Mitf antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 3-5 ug/ml
• Immunogen: Synthetic peptide corresponding to amino acids 301-312 of human Mitf.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of Mitf using IMG-488 at 1:1000 dilution against A375 (human, lane 1) and at 1:250 dilution against 10 ug of Raw (mouse, lane 2) cell lysates.)

Related Product Information for anti-Mitf antibody

Microphthalmia-associated bHLH-LZ transcription factor (Mitf) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC transcription factors. Mitf can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. Mitf is expressed as multiple isoforms, which share common transcription activation and DNA-binding domains, but differ in the amino-terminal region. Mitf is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases.

Product Categories/Family for anti-Mitf antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 3319231
• NCBI GeneID: 25094
• UniProt Accession #: O88368
• Molecular Weight: 13,155 Da
• NCBI Official Full Name: microphthalmia associated transcription factor
• NCBI Official Synonym Full Names: microphthalmia-associated transcription factor
• NCBI Official Symbol: Mitf
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: Mitf
• UniProt Entry Name: MITF_RAT

NCBI Description

basic helix-loop-helix-leucine zipper transcription factor; may play a role in osteoclast function [RGD, Feb 2006]

Uniprot Description

MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Cellular Component: protein complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; camera-type eye development; cell fate commitment; positive regulation of transcription, DNA-dependent; osteoclast differentiation; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; regulation of cell proliferation; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; pigmentation; regulation of gene expression; melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; cell differentiation; negative regulation of apoptosis