Testing Data
(Detection of Prx-1 with MBS606107. Lysates from human Raji, A431 and MCF-7 cells were resolved by SDS-PAGE, transferred to Immobilon membranes and immunoblotted with 0.1ug/ml Peroxiredoxin 1.)
Mouse anti-Human Peroxiredoxin 1 Monoclonal Antibody | anti-RB12362 antibody
Peroxiredoxin 1 (PRDX1)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Peroxiredoxin 1; Monoclonal Antibody; Peroxiredoxin 1 (PRDX1); Anti -Peroxiredoxin 1 (PRDX1); anti-RB12362 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9j9
Specificity
Recognizes human Peroxiredoxin 1. Species sequence homology: mouse (96%).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide.
Applicable Applications for anti-RB12362 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 0.1ug/ml
Dilution: Western Blot: 0.1ug/ml
Immunogen
E. coli-derived, recombinant human Peroxiredoxin 1 (aa1-199).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of Prx-1 with MBS606107. Lysates from human Raji, A431 and MCF-7 cells were resolved by SDS-PAGE, transferred to Immobilon membranes and immunoblotted with 0.1ug/ml Peroxiredoxin 1.)
Testing Data
(Detection of Prx-1 with MBS606107. Lysates from human Raji, A431 and MCF-7 cells were resolved by SDS-PAGE, transferred to Immobilon membranes and immunoblotted with 0.1ug/ml Peroxiredoxin 1.)
Related Product Information for anti-RB12362 antibody
Human Peroxiredoxin1 (Prx-1, also known as Thioredoxin peroxidase 2) is a 22kD antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199aa in length and has two catalytic cysteines, one at Cys52 and a second at Cys173. Prx-1 is an obligate homodimer. In its inactive state, Prx-1 is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms; one shows a premature truncation after aa171, while the second shows a deletion of aa21-121. Human Prx-1 is 96% aa identical to mouse Prx-1.
Product Categories/Family for anti-RB12362 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
peroxiredoxin
NCBI Official Symbol
RB12362
NCBI Protein Information
peroxiredoxin
Protein Family
Similar Products
Product Notes
The RB12362 (Catalog #AAA606107) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Peroxiredoxin 1 (PRDX1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.1ug/ml. Researchers should empirically determine the suitability of the RB12362 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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