Sheep anti-Human Draxin Polyclonal Antibody | anti-DRAXIN antibody

Draxin (Dorsal Repulsive Axon Guidance Protein, Neucrin, C1orf187, PSEC0258, UNQ3119/PRO10268)

Cat. Num. AAA629987

• Gene Names: DRAXIN; UNQ3119; neucrin; AGPA3119; C1orf187
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Draxin; Polyclonal Antibody; Draxin (Dorsal Repulsive Axon Guidance Protein; Neucrin; C1orf187; PSEC0258; UNQ3119/PRO10268); Anti -Draxin (Dorsal Repulsive Axon Guidance Protein; anti-DRAXIN antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Draxin. Species sequence homology: mouse (79%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-DRAXIN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1ug/ml; ELISA: 0.5-1ug/ml
• Immunogen: Purified, NS0-derived, recombinant human Draxin (aa 26 - 349; Accession # Q8NBI3).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of Draxin: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/mL sheep anti-hDraxin.)

Related Product Information for anti-DRAXIN antibody

Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58kD, presumable glycoprotein member of the draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature human Draxin is 324aa in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa274-333).

Product Categories/Family for anti-DRAXIN antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 86990448
• NCBI GeneID: 374946
• NCBI Accession #: NP_940947.3
• NCBI GenBank Nucleotide #: NM_198545.3
• UniProt Accession #: Q8NBI3; Q5SNX0; Q6UWK8; B6EV15
• Molecular Weight: 38,650 Da
• NCBI Official Full Name: draxin
• NCBI Official Synonym Full Names: dorsal inhibitory axon guidance protein
• NCBI Official Symbol: DRAXIN
• NCBI Official Synonym Symbols: UNQ3119; neucrin; AGPA3119; C1orf187
• NCBI Protein Information: draxin; dorsal repulsive axon guidance protein; neural tissue-specific cysteine-rich protein
• UniProt Protein Name: Draxin
• Protein Family: Draxin
• UniProt Gene Name: DRAXIN
• UniProt Synonym Gene Names: C1orf187
• UniProt Entry Name: DRAXI_HUMAN

Uniprot Description

DRAXIN: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta- catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway. Belongs to the draxin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: extracellular region

Biological Process: dorsal spinal cord development; axon guidance; Wnt receptor signaling pathway; commissural neuron differentiation in the spinal cord; forebrain development; negative regulation of axon extension; negative regulation of neuron apoptosis