Rabbit anti-Human Kallikrein-3 Polyclonal Antibody | anti-KLK3 antibody

Anti-Kallikrein-3 (Prostate Specific Antigen (PSA), APS), Catalytic Domain Antibody

Cat. Num. AAA4157515

• Gene Names: KLK3; PSA
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Kallikrein-3; Polyclonal Antibody; Anti-Kallikrein-3 (Prostate Specific Antigen (PSA); APS); Catalytic Domain Antibody; anti-KLK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for Kallikrein 3, it does not react with the other Kallikreins (1,2,4-15).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 180

• Applicable Applications for anti-KLK3 antibody: Western Blot (WB)
• Application Notes: SDS-PAGE: Use at an assay dependent dilution. Glycosylation and other post translational modifications make the apparent weight on SDS PAGE gels 32-33 kDa.; WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 29 kDa. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the catalytic domain of human Kallikrein 3.
• Cellular Localization: Cytoplasmic and Secreted
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-KLK3 antibody

Description: The antibody is a rabbit polyclonal antibody made to the serine proteinase kallikrein-3 (PSA, hK3). The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-3. The antibody reacts with human Kallikrein-3, and does not react with the human kallikreins (hK1, 3-15), although hK2 can be detected in slightly. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Kallikrein-3, also known as Prostate Specific Antigen (PSA) was first discovered as a major protein in semen, and determined to be a prostate-specific protein. Since then, other tissues and bodily fluids have been shown to contain Kallikrein-3, but at much lower levels than in seminal plasma. Kallikrein-3 is a serine proteinase with trypsin-chymotrypsin specificities, thought to be important in liquefying clots in semen. The active site has a catalytic triad of His-Asp-Ser, with the serine in a Gly-X-Ser-X-Gly arrangement. The utility of Kallikrein-3 as a prostate-specific marker has allowed its use for assays of prostate cancer. Both Kallikrein-3 and Kallikrein-2 are produced by the prostate, but the blood-testis barrier usually keeps the circulating levels low. Prostate tumors or benign prostate hyperplasia give Kallikrein-3 access to normal circulation, and measurement of the PSA levels is a reasonable indicator of the amount of access. It was thought that Kallikrein-3 was responsible for allowing or making the tumor cells to spread aggressively, but it is still unclear what if any role Kallikrein-3 plays in tumor biology. Pre-pro-kallikrein-3 is processed to a 237 amino acid long active enzyme, with amino terminal sequence IVGGWE. Kallikrein-3 forms complexes with and is inhibited by a number of endogenous proteinase inhibitors, including beta1-Protienase Inhibitor and protein C inhibitor.The full length message for Kallikrein-3 encodes a protein of 261 amino acids, with a predicted mass of 28.74 kDa and a pI of 8.27. Gllcosylation and other post translational modifications make the apparent weight on SDS PAGE gels 32-33 kDa, The pI is significantly more basic than for Kallikrein-1 and Kallikrein-2, which have predicted pI of 4.54 and 6.49, respectively. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the identification and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 57114133
• NCBI GeneID: 493182
• NCBI GenBank Nucleotide #: NP_001009136.1
• NCBI Official Full Name: kallikrein 3
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: PSA
• NCBI Protein Information: kallikrein 3
• Protein Family: Glandular kallikrein