Rabbit anti-Human Kallikrein-11 Polyclonal Antibody | anti-KLK11 antibody

Anti-Kallikrein-11 (Hippostasin, TLSP, PRSS20), Catalytic Domain Antibody

Cat. Num. AAA4157531

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Kallikrein-11; Polyclonal Antibody; Anti-Kallikrein-11 (Hippostasin; TLSP; PRSS20); Catalytic Domain Antibody; anti-KLK11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody reacts with human Kallikrein 11 and does not react with the human kallikreins (hK1-10, 12-15).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-KLK11 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 31 kDa. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the catalytic domain of human Kallikrein 11.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-KLK11 antibody

Description: The antibody is a rabbit polyclonal antibody made to the serine proteinase kallikrein-11 (hK11). The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-11. The antibody reacts with human Kallikrein-11, and does not react with the human kallikreins (hK1-15). The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Kallikrein-11, also known as hippostasin (hippocampus and prostate trypsin), PRSS20 (protease serine-20) and TLSP (trypsin-like serine protease), is a trypsin-like serine proteinase. Originally described from the hippocampus area of the brain and from the prostate, Kallikrein-11 is also produced in the lung, pancreas, skin, testis, heart, liver, stomach and salivary glands. Biological fluids such as breast milk, serum and seminal plasma have also been shown to contain Kallikrein-11. Kallikrein-11 has been reported to be elevated in prostate cancer, ovarian cancer and colon cancer, and the ratio of hK11 to hK3 in sera from prostate cancer patients was lower than sera from patients with benign prostatic hyperplasia, suggesting that Kallikrein-11 might be used fine-tune PSA assays. Many cell lines express Kallikrein-11 in culture, and like many of the kallikreins, the production is stimulated by hormones. Different splice variants have been identified; a prostate-type of 282 amino acids, a brain-type of 250 amino acids, and a variant type of 275 amino acids. The prostate form has an extended aminoterminus, and the variant form a longer catalytic domain, relative to the brain type. Endogenous inhibitors include kallistatin, protein-C inhibitor and beta1-proteinase inhibitor, although Kallikrein-11 can be found complexed to a number of different proteinase inhibitors. The longest Kallikrein-11 sequence codes for a protein of 282 amino acids, with predicted mass of 31.1 kDa, and a pI of 10.0, the 275 amino acid version is predicted to be 30.2 kDa and 9.46 pI, and the 250 amino acid form 27.5 kDa with 9.6 pI. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 11 has two alternative splicing isoforms, known as the brain type and prostate type. Elevated serum levels of hK11 have been found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of this biomarker in serum may aid in the identification and monitoring of ovarian and prostatic carcinoma.

NCBI and Uniprot Product Information

• NCBI GI #: 262527289
• NCBI GenBank Nucleotide #: NP_001161077.1
• UniProt Accession #: Q9UBX7
• NCBI Official Full Name: kallikrein-11 isoform 3
• UniProt Protein Name: Kallikrein-11
• Protein Family: Kallikrein
• UniProt Gene Name: KLK11
• UniProt Synonym Gene Names: PRSS20; TLSP; hK11
• UniProt Entry Name: KLK11_HUMAN

Uniprot Description

KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted; EC 3.4.21.-; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis