Testing Data
Rabbit anti-Human Kallikrein-11 Polyclonal Antibody | anti-KLK11 antibody
Anti-Kallikrein-11 (Hippostasin, TLSP, PRSS20), Kallikrein Loop Antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Kallikrein-11; Polyclonal Antibody; Anti-Kallikrein-11 (Hippostasin; TLSP; PRSS20); Kallikrein Loop Antibody; anti-KLK11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody reacts with human Kallikrein 11, and does not react with other human kallikreins (hK1-15).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
275
Applicable Applications for anti-KLK11 antibody
Western Blot (WB)
Application Notes
WB: 1:1000. Predicted molecular weight: 31 kDa. Notes: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.
Immunogen
Synthetic peptide (Human), based on the Kallikrein loop area of human Kallikrein 11.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-KLK11 antibody
Description: The antibody is a rabbit polyclonal antibody made to the serine proteinase kallikrein-11 (hK11). The antibody is made to a synthetic peptide based on the Kallikrein loop area of human Kallikrein-11. The antibody reacts with human Kallikrein-11, and does not react with the human kallikreins (hK1-15). The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Kallikrein-11, also known as hippostasin (hippocampus and prostate trypsin), PRSS20 (protease serine-20) and TLSP (trypsin-like serine protease), is a trypsin-like serine proteinase. Originally described from the hippocampus area of the brain and from the prostate, Kallikrein-11 is also produced in the lung, pancreas, skin, testis, heart, liver, stomach and salivary glands. Biological fluids such as breast milk, serum and seminal plasma have also been shown to contain Kallikrein-11. Kallikrein-11 has been reported to be elevated in prostate cancer, ovarian cancer and colon cancer, and the ratio of hK11 to hK3 in sera from prostate cancer patients was lower than sera from patients with benign prostatic hyperplasia, suggesting that Kallikrein-11 might be used fine-tune PSA assays. Many cell lines express Kallikrein-11 in culture, and like many of the kallikreins, the production is stimulated by hormones. Different splice variants have been identified; a prostate-type of 282 amino acids, a brain-type of 250 amino acids, and a variant type of 275 amino acids. The prostate form has an extended aminoterminus, and the variant form a longer catalytic domain, relative to the brain type. Endogenous inhibitors include kallistatin, protein-C inhibitor and beta1-proteinase inhibitor, although Kallikrein-11 can be found complexed to a number of different proteinase inhibitors. The longest Kallikrein-11 sequence codes for a protein of 282 amino acids, with predicted mass of 31.1 kDa, and a pI of 10.0, the 275 amino acid version is predicted to be 30.2 kDa and 9.46 pI, and the 250 amino acid form 27.5 kDa with 9.6 pI. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Relevance: The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 11 has two alternative splicing isoforms, known as the brain type and prostate type. Elevated serum levels of hK11 have been found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of this biomarker in serum may aid in the identification and monitoring of ovarian and prostatic carcinoma.
Relevance: The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 11 has two alternative splicing isoforms, known as the brain type and prostate type. Elevated serum levels of hK11 have been found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of this biomarker in serum may aid in the identification and monitoring of ovarian and prostatic carcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kallikrein-11 isoform 3
UniProt Protein Name
Kallikrein-11
Protein Family
UniProt Gene Name
KLK11
UniProt Synonym Gene Names
PRSS20; TLSP; hK11
UniProt Entry Name
KLK11_HUMAN
Uniprot Description
KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Secreted; EC 3.4.21.-; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: Golgi apparatus; extracellular space
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
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Product Notes
The KLK11 klk11 (Catalog #AAA4157530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kallikrein-11 (Hippostasin, TLSP, PRSS20), Kallikrein Loop Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein-11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Predicted molecular weight: 31 kDa. Notes: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Researchers should empirically determine the suitability of the KLK11 klk11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein-11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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