Pig Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit | PPARGC1A elisa kit

Pig Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit

Cat. Num. AAA288484

• Gene Names: PPARGC1A; PGC1; PGC1A; PPARGC1; PPARGC-1
• Reactivity: Pig
• Synonyms: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Pig Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PPARGC1A elisa kit

• Reactivity: Pig
• Specificity: This assay recognizes recombinant and natural pig PGC-1-alpha. No significant cross-reactivity or interference was observed.
• Sequence Length: 798

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.15 ng/ml -10.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of pig PGC-1-alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPARGC1A elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGC-1-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGC-1-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PGC-1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PGC-1-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 697663041
• NCBI GeneID: 397013
• NCBI Accession #: NP_999128.2
• NCBI GenBank Nucleotide #: NM_213963.2
• UniProt Accession #: Q865B6
• Molecular Weight: 90,336 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• NCBI Official Symbol: PPARGC1A
• NCBI Official Synonym Symbols: PGC1; PGC1A; PPARGC1; PPARGC-1
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• Protein Family: Peroxisome proliferator-activated receptor gamma coactivator
• UniProt Gene Name: PPARGC1A
• UniProt Synonym Gene Names: PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
• UniProt Entry Name: PRGC1_PIG

Uniprot Description

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK ().