Rabbit anti-Human Kallikrein-13 (KLK-L4) Polyclonal Antibody | anti-KLK13 antibody

Anti-Kallikrein-13 (KLK-L4), Catalytic Domain Antibody

Cat. Num. AAA4157535

• Gene Names: KLK13; KLKL4; KLK-L4
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Kallikrein-13 (KLK-L4); Polyclonal Antibody; Anti-Kallikrein-13 (KLK-L4); Catalytic Domain Antibody; anti-KLK13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody reacts with human Kallikrein 13 and does not react with the human kallikreins (hK1-12, 14-15).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 125

• Applicable Applications for anti-KLK13 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 31 kDa. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the catalytic domain of human Kallikrein 13.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-KLK13 antibody

Description: The antibody is a rabbit polyclonal antibody made to the serine proteinase kallikrein-13 (hK13). The antibody is made to a synthetic peptide based on the catalytic domain of human Kallikrein-13. The antibody reacts with human Kallikrein-13, and does not react with the human kallikreins (hK1-15). The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Kallikrein-13, also known as Kallikrein-like4 (KLK-L4), is a trypsin-like serine proteinase. Kallikrein-13 was discovered by cloning the kallikrein locus on chromosome 19, and was found to be similar to the other kallikrein family genes. A mouse Kallikrein, mGK-13, the prorenin converting enzyme, is not the species ortholog of kallikrein-13. Kallikrein-13 is produced by a number of tissues, including the esophagus, tonsils, pancreas, salivary gland, breast, prostate, uterus, lung, thymus, thyroid, brain, stomach and colon. Like many of the kallikreins, Kallikrein-13 is also found in biological fluids like serum, breast milk, amniotic fluid, seminal plasma and cerebrospinal fluid. Kallikrein-13 message has been reported to be decreased in some breast cancers, and in ovarian cancers, similar to Kallikrein-10. Some cell lines express Kallikrein-13 in culture, and like many of the kallikreins, the production is stimulated by hormones. Different splice variants of kallikrein-13 have been identified; a 277 amino acid form, a 204 amino acid form, and a truncated 125 amino acid form. The differences are all in the amino end of the kallikrein-13 catalytic domain, with the 204 amino acid form missing the aspartic acid and the 125 amino acid form missing the aspartic acid and histidine of the catalytic triad. These two shorter forms of Kallikrein-13 are not predicted to be proteolytically active. Likewise, in the testis a number of truncated forms of Kallikrein-13 message have been identified, but it is unclear if any of these shorter sequences are produced as protein. Endogenous inhibitors include kallistatin, protein-C inhibitor and beta1-proteinase inhibitor, although Kallikrein-13 can be found complexed to a number of different proteinase inhibitors. The longest Kallikrein-13 sequence codes for a protein of 277 amino acids, with predicted mass of 30.6 kDa, and a pI of 9.4. The 204 amino acid version is predicted to be 22.4 kDa and 9.54 pI. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp, and Ser residues, typically five coding exons, and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 13, also known as Kallikrein-like4 (KLK-L4), is a trypsin-like serine proteinase. It was discovered by cloning the kallikrein locus on chromosome 19 and was found to be similar to the other kallikrein family genes.

NCBI and Uniprot Product Information

• NCBI GI #: 1131345312
• NCBI GeneID: 26085
• NCBI GenBank Nucleotide #: NP_001335107.1
• UniProt Accession #: Q9UKR3
• NCBI Official Full Name: kallikrein-13 isoform 3
• NCBI Official Synonym Full Names: kallikrein related peptidase 13
• NCBI Official Symbol: KLK13
• NCBI Official Synonym Symbols: KLKL4; KLK-L4
• NCBI Protein Information: kallikrein-13
• UniProt Protein Name: Kallikrein-13
• Protein Family: Kallikrein
• UniProt Gene Name: KLK13
• UniProt Synonym Gene Names: KLKL4; KLK-L4
• UniProt Entry Name: KLK13_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Expression of this gene is regulated by steroid hormones and may be useful as a marker for breast cancer. [provided by RefSeq, Jan 2017]

Uniprot Description

KLK13: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Expression of this gene is regulated by steroid hormones and may be useful as a marker for breast cancer. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: hydrolase activity; protein binding; serine-type endopeptidase activity

Biological Process: protein processing