Testing Data
Rabbit anti-Human Kallikrein-10 Polyclonal Antibody | anti-KLK10 antibody
Anti-Kallikrein-10 (NES1, PRSSL1), Kallikrein Loop Antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Kallikrein-10; Polyclonal Antibody; Anti-Kallikrein-10 (NES1; PRSSL1); Kallikrein Loop Antibody; anti-KLK10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody reacts with human Kallikrein 10, and does not react with the human kallikreins (hK1-15).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
276
Applicable Applications for anti-KLK10 antibody
Western Blot (WB)
Application Notes
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 30 kDa. Dilution optimised using Chromogenic detection.
Immunogen
Synthetic peptide based on the Kallikrein loop area of human Kallikrein 10.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-KLK10 antibody
Description: The antibody is a rabbit polyclonal antibody made to the serine proteinase kallikrein-10 (hK10). The antibody is made to a synthetic peptide based on the Kallikrein loop area of human Kallikrein-10. The antibody reacts with human Kallikrein-10, and does not react with the human kallikreins (hK1-15). The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Kallikrein-10, also known as normal epithelial cell specific-1 (NES1) and protease serine-like 1 (PRSSL1), is a trypsin-like serine proteinase. Originally described from a subtractive library looking for genes that were down-regulated in breast cancer, kallikrein-10 was thought to be epithelial cell specific. Kallikrein-10 is reported to be up regulated in pancreatic cancer and ovarian cancer, but down regulated in testicular cancer and breast cancer, perhaps due to differential hormonal regulation, or tissue specificities. Breast cancer patients that had elevated kallikrein-10 had a worse prognosis, leading to speculation that kallikrein-10 might be protective in breast cancer, similar to the argument put forth for kallikrein-3 in prostate cancer. Kallikrein-10 is found in high levels in the pancreas, and in lower levels in the tonsils, esophagus, skin, brain, testis and ovary. Biological fluids (breast milk, serum, seminal plasma, urine) contain kallikrein-10, the highest levels found in milk. The cerebral spinal fluid (CSF) contains Kallikrein-10, and was reported to be elevated in patients with Alzheimer's disease. Endogenous inhibitors include kallistatin, protein-C inhibitor and beta1-proteinase inhibitor, although Kallikrein-10 can be found complexed to a number of different proteinase inhibitors. Pre-pro-kallikrein-10 has the 33 amino acid signal sequence is removed before secretion, and the Pro-kallikrein-10 is activated to Kallikrein-10 by removal of the 10 amino acid propeptide domain. The full length Kallikrein-10 sequence codes for a protein of 276 amino acids, with predicted mass of 30.1kDa, and a pI of 9.78. A range of different sized bands can be seen for Kallikrein-10 in different tissues and in cell culture media, ranging from 16-34 kDa, and likely representing different proteolytically processed forms. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Relevance: Kallikrein 10, also known as normal epithelial cell specific-1 (NES1) and protease serine-like 1 (PRSSL1), is a trypsin-like serine proteinase. Originally described from a subtractive library looking for genes that were down-regulated in breast cancer, kallikrein 10 was thought to be epithelial cell specific. Kallikrein 10 is reported to be up regulated in pancreatic cancer and ovarian cancer, but down regulated in testicular cancer and breast cancer, perhaps due to differential hormonal regulation, or tissue specificities. Breast cancer patients that had elevated kallikrein 10 had a worse prognosis, leading to speculation that kallikrein 10 might be protective in breast cancer, similar to the argument put forth for kallikrein 3 in prostate cancer. Kallikrein 10 is found in high levels in the pancreas and in lower levels in the tonsils, esophagus, skin, brain, testis and ovary. Biological fluids (breast milk, serum, seminal plasma, urine) contain kallikrein 10, the highest levels found in milk. The cerebral spinal fluid (CSF) contains Kallikrein 10, and was reported to be elevated in patients with Alzheimer's disease. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 10 can be found complexed to a number of different proteinase inhibitors.
Relevance: Kallikrein 10, also known as normal epithelial cell specific-1 (NES1) and protease serine-like 1 (PRSSL1), is a trypsin-like serine proteinase. Originally described from a subtractive library looking for genes that were down-regulated in breast cancer, kallikrein 10 was thought to be epithelial cell specific. Kallikrein 10 is reported to be up regulated in pancreatic cancer and ovarian cancer, but down regulated in testicular cancer and breast cancer, perhaps due to differential hormonal regulation, or tissue specificities. Breast cancer patients that had elevated kallikrein 10 had a worse prognosis, leading to speculation that kallikrein 10 might be protective in breast cancer, similar to the argument put forth for kallikrein 3 in prostate cancer. Kallikrein 10 is found in high levels in the pancreas and in lower levels in the tonsils, esophagus, skin, brain, testis and ovary. Biological fluids (breast milk, serum, seminal plasma, urine) contain kallikrein 10, the highest levels found in milk. The cerebral spinal fluid (CSF) contains Kallikrein 10, and was reported to be elevated in patients with Alzheimer's disease. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 10 can be found complexed to a number of different proteinase inhibitors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kallikrein-10 preproprotein
UniProt Protein Name
Kallikrein-10
Protein Family
UniProt Gene Name
KLK10
UniProt Synonym Gene Names
NES1; PRSSL1
UniProt Entry Name
KLK10_HUMAN
Uniprot Description
KLK10: Has a tumor-suppressor role for NES1 in breast and prostate cancer. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: Tumor suppressor; Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: extracellular region
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis; cell cycle
Similar Products
Product Notes
The KLK10 klk10 (Catalog #AAA4157528) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kallikrein-10 (NES1, PRSSL1), Kallikrein Loop Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein-10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 30 kDa. Dilution optimised using Chromogenic detection. Researchers should empirically determine the suitability of the KLK10 klk10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein-10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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