Testing Data
Rabbit anti-Human Kallikrein-6 Polyclonal Antibody | anti-KLK6 antibody
Anti-Kallikrein-6 (Zyme, Neurosin, PRSS9, Protease M, Myelencephalon-Specific Protease (MSP)), Catalytic Domain Antibody
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Relevance: Kallikreins (KLKs) are a subgroup of trypsin-like serine proteases and are implicated in carcinogenesis. Originally described from human urine as a hypotensive agent and later from the pancreas, kallkreins were given the Greek name for pancreas, kallikreas. Kallikrein 6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein 6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP) and hK6 was thought to be a beta secretase. Kallikrein 6 was shown to be elevated in the sera patients with Alzheimer's disease and Parkinson's disease and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin. Kallikrein 6 is found in greatest abundance in the brain and neuronal tissues, with lesser levels in the kidney, thymus, spleen and lymph nodes. Human breast milk is reported to contain high levels of Kallikrein 6 and hK6 has been purified from cerebral spinal fluid (CSF). Less stringent PCR detects hK6 in many tissues and analysis of cell lines by Western blot shows that many cell lines make hK6. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 6 can be found complexed to a number of different proteinase inhibitors.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the kallikrein subfamily of the peptidase S1 family of serine proteases. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The encoded preproprotein is proteolytically processed to generate the mature protease. Expression of this protease is regulated by steroid hormones and may be elevated in multiple human cancers and in serum from psoriasis patients. The encoded protease may participate in the cleavage of amyloid precursor protein and alpha-synuclein, thus implicating this protease in Alzheimer's and Parkinson's disease, respectively. This gene is located in a gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Uniprot Description
KLK6: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.21.-; Secreted, signal peptide; Nucleolus; Secreted
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; microtubule cytoskeleton; extracellular space; intercellular bridge; nuclear membrane; mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus
Molecular Function: protein binding; serine-type endopeptidase activity
Biological Process: myelination; collagen catabolic process; protein autoprocessing; central nervous system development; amyloid precursor protein metabolic process; tissue regeneration; positive regulation of G-protein coupled receptor protein signaling pathway; regulation of cell differentiation; response to wounding; hormone metabolic process; protein processing; proteolysis
Research Articles on KLK6
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Product Notes
The KLK6 klk6 (Catalog #AAA4157521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kallikrein-6 (Zyme, Neurosin, PRSS9, Protease M, Myelencephalon-Specific Protease (MSP)), Catalytic Domain Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein-6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 (when using colorimetric substrates such as BCIP/NBT)-1:5000 (for chemiluminescent substrates). Predicted molecular weight: 26.9 kDa. The processed hK6 has an apparent mass of 31-33 kDa on reduced SDS PAGE gels. A range of different sized bands can be seen for Kallikrein-6 in different tissues and in cell culture media, ranging from 16-32 kDa, probably representing different proteolytically processed forms but perhaps also the different splice variants. Dilution optimised using Chromogenic detection. Researchers should empirically determine the suitability of the KLK6 klk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein-6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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