Rabbit anti-Human Kallikrein-7 Polyclonal Antibody | anti-KLK7 antibody

Anti-Kallikrein-7 (Stratum Corneum Chymotryptic Enzyme (SCCE), PRSS6), Kallikrein Loop Antibody

Cat. Num. AAA4157522

• Gene Names: KLK7; hK7; SCCE; PRSS6
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Kallikrein-7; Polyclonal Antibody; Anti-Kallikrein-7 (Stratum Corneum Chymotryptic Enzyme (SCCE); PRSS6); Kallikrein Loop Antibody; anti-KLK7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody reacts with Kallikrein-7, and does not react with kallikreins (hK1-15).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 253

• Applicable Applications for anti-KLK7 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000. Predicted molecular weight: 28 kDa. Notes: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide of Human Kallikrein 7 based on the Kallikrein loop area.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-KLK7 antibody

Description: The antibody is a rabbit polyclonal antibody made to the serine proteinase kallikrein-7 (hK7). The antibody is made to a synthetic peptide based on the Kallikrein loop area of human Kallikrein-7. RP1-hK7 reacts with human Kallikrein-7, and does not react with the human kallikreins (hK1-15). The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Kallikrein-7, also known as stratum corneum chymotryptic enzyme (SCCE) and PRSS6, is a chymotrypsin-like serine proteinase. Originally described from human skin as a serine protease involved in shedding of skin cells and remodeling if the skin, SCCE was later identified as Kallikrein-7. Kallikrein-7 is found at the highest levels in the skin, often complexed with the endogenous serpins SLPI or elafin, and kallikrein-7 can be found complexed to a number of different proteinase inhibitors. In addition to skin, Kallikrein-7 has been found in the kidney, esophagus, neuronal tissues, amniotic fluid, saliva, breast milk, urine, synovial fluid, seminal plasma and serum. Kallikrein-7 has been reported to be decreased in the CSF of Alzheimer's patients, and message levels of KLK7 were decreased in adenocarcinoma. In skin, overexpression of hK7 has been shown to cause a form of dermatitis, and in psoriasis hK7 is expressed in higher levels than controls. The skin adhesive proteins corneodesmosin and desmocollin 1 have been reported to be substrates of Kallikrein-7, as is interleukin-1beta and the insulin B-chain. Splice variants of Kallikrein-7 have been suggested as markers in ovarian cancer, and hK7 is reported to be elevated in squamous cervical cancer cells. Kallikrein-7 gene expression has been reported to be hormonally regulated, increased by estrogen in breast cancer cells in culture. Pre-pro-kallikrein-7 has the 22 amino acid signal sequence is removed before secretion, and the Pro-kallikrein-7 is activated to Kallikrein-7 by removal of the 7 amino acid propeptide domain. The full length Kallikrein-7 sequence codes for a protein of 253 amino acids, with predicted mass of 27.6 kDa, and a pI of 9.49. A shorter sequence starting at the second methionine has been reported, the 181 amino acid protein has a predicted mass of 19.9 kDa and a pI of 9.67. This shorter sequence, if produced as a protein, would lack the cysteine of the catalytic triad, and likely be proteolytically inactive. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp, and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N-terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer. Kallikrein 7, also known as stratum corneum chymotryptic enzyme (SCCE) and PRSS6, is a chymotrypsin-like serine proteinase. Originally described from human skin as a serine protease involved in shedding of skin cells and remodeling if the skin, SCCE was later identified as Kallikrein 7. Kallikrein 7 is found at the highest levels in the skin, often complexed with the endogenous serpins SLPI or elafin and kallikrein 7 can be found complexed to a number of different proteinase inhibitors. In addition to skin, Kallikrein 7 has been found in the kidney, esophagus, neuronal tissues, amniotic fluid, saliva, breast milk, urine, synovial fluid, seminal plasma and serum. Kallikrein 7 has been reported to be decreased in the CSF of Alzheimer's patients and message levels of KLK7 were decreased in adenocarcinoma. In skin, overexpression of hK7 has been shown to cause a form of dermatitis and in psoriasis hK7 is expressed in higher levels than controls. The skin adhesive proteins corneodesmosin and desmocollin 1 have been reported to be substrates of Kallikrein 7, as is interleukin 1 and the insulin B-chain.

NCBI and Uniprot Product Information

• NCBI GI #: 21327705
• NCBI GeneID: 5650
• NCBI GenBank Nucleotide #: NP_644806.1
• UniProt Accession #: P49862
• NCBI Official Full Name: kallikrein-7 isoform 1 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 7
• NCBI Official Symbol: KLK7
• NCBI Official Synonym Symbols: hK7; SCCE; PRSS6
• NCBI Protein Information: kallikrein-7
• UniProt Protein Name: Kallikrein-7
• Protein Family: Kallikrein
• UniProt Gene Name: KLK7
• UniProt Synonym Gene Names: PRSS6; SCCE; hK7; hSCCE
• UniProt Entry Name: KLK7_HUMAN

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases. These enzymes have diverse physiological functions and many kallikrein genes are biomarkers for cancer. The encoded protein has chymotrypsin-like activity and plays a role in the proteolysis of intercellular cohesive structures that precedes desquamation, the shedding of the outermost layer of the epidermis. The encoded protein may play a role in cancer invasion and metastasis, and increased expression of this gene is associated with unfavorable prognosis and progression of several types of cancer. Polymorphisms in this gene may play a role in the development of atopic dermatitis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, which is one of fifteen kallikrein subfamily members located in a gene cluster on chromosome 19. [provided by RefSeq, May 2011]

Uniprot Description

KLK7: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. SCCE cleaves insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.35; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; epidermis development; proteolysis