Rabbit anti-Human Integrin, beta5 Polyclonal Antibody | anti-Itgb5 antibody

Integrin, beta5

Cat. Num. AAA617750

• Gene Names: Itgb5; [b]5; [b]-5; [b]5A; [b]5B; beta5; ESTM23; beta-5; AA475909; AI874634
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Integrin; beta5; Polyclonal Antibody; Anti -Integrin; anti-Itgb5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total human integrin b5 protein at ~90kD.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA. 50% glycerol.

• Applicable Applications for anti-Itgb5 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Dilution: Western Blot: 1:1000; Immunofluorescence (IC): 1:200; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide corresponding to residues surrounding Lys789 of human integrin b5 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HT29 and A172 cell lines using MBS617750.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of A172 cells using MBS617750 (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-Itgb5 antibody

Integrins are a/b heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 a and 8 b subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). The aVb5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). aVb5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the aVb5 integrin and growth factors regulates endothelial cell proliferation and survival (8).

Product Categories/Family for anti-Itgb5 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 225007621
• NCBI GeneID: 16419
• NCBI Accession #: NP_001139356.1
• NCBI GenBank Nucleotide #: NM_001145884.1
• UniProt Accession #: P18084; O70309; Q6PE70
• Molecular Weight: 90kD
• NCBI Official Full Name: integrin beta-5 isoform 1
• NCBI Official Synonym Full Names: integrin beta 5
• NCBI Official Symbol: Itgb5
• NCBI Official Synonym Symbols: [b]5; [b]-5; [b]5A; [b]5B; beta5; ESTM23; beta-5; AA475909; AI874634
• NCBI Protein Information: integrin beta-5
• UniProt Protein Name: Integrin beta
• Protein Family: Integrin
• UniProt Gene Name: Itgb5
• UniProt Entry Name: Q6PE70_MOUSE

Uniprot Description

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: focal adhesion; cell surface; membrane; leading edge; integral to membrane; integrin complex; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity; receptor binding

Biological Process: integrin-mediated signaling pathway; cell-matrix adhesion; transforming growth factor beta receptor signaling pathway; multicellular organismal development; stress fiber formation; cell adhesion