Rabbit RRAGC Monoclonal Antibody | anti-RRAGC antibody

RRAGC (Ras-related GTP Binding C, AU041672, FLJ13311, GTPase-interacting Protein 2, GTR2, MGC47404, OTTHUMP00000000548, Rag C Protein, RAGC, RP23-29H22.4, TIB929, YGR163W)

Cat. Num. AAA607136

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: RRAGC; Monoclonal Antibody; RRAGC (Ras-related GTP Binding C; AU041672; FLJ13311; GTPase-interacting Protein 2; GTR2; MGC47404; OTTHUMP00000000548; Rag C Protein; RAGC; RP23-29H22.4; TIB929; YGR163W); Anti -RRAGC (Ras-related GTP Binding C; anti-RRAGC antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10g90
• Specificity: Recognizes endogenous levels of total RagC protein. Species crossreactivity: Human, mouse, rat and monkey
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-RRAGC antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunofluorescence.; Dilution: Immunofluorescence: 1:100; Flow Cytometry: 1:200; Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues in the amino terminus of human RagC.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using MBS607136.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HeLa MBS607136 (blue) compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS607136 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)

Related Product Information for anti-RRAGC antibody

mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

Product Categories/Family for anti-RRAGC antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 115497870
• NCBI GeneID: 617367
• NCBI Accession #: NP_001069924.1
• NCBI GenBank Nucleotide #: NM_001076456.1
• NCBI Official Full Name: ras-related GTP-binding protein C
• NCBI Official Synonym Full Names: Ras-related GTP binding C
• NCBI Official Symbol: RRAGC
• NCBI Protein Information: ras-related GTP-binding protein C
• Protein Family: Ras-related GTP-binding protein