Western Blot (WB)
(Western blot analysis of extracts from 293, HeLa, Jurkat and COS cells using MBS616581.)
Rabbit anti-Human OSR1 Antibody | anti-OXSR1 antibody
OSR1 (Oxidative Stress Responsive 1)
Flow Cytometry: 1:100
Immunofluorescence (IC): 1:25
Optimal dilutions to be determined by the researcher.
Other applications not tested.
Western Blot (WB)
(Western blot analysis of extracts from 293, HeLa, Jurkat and COS cells using MBS616581.)
Western Blot (WB)
(Western blot analysis of extracts from 293, HeLa, Jurkat and COS cells using MBS616581.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using MBS616581 (blue) compared to a nonspecific negative control antibody (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using MBS616581 (blue) compared to a nonspecific negative control antibody (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using MBS616581 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using MBS616581 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq]
Uniprot Description
OSR1: oxidative-stress responsive 1 protein is a ubiquitously expressed protein kinase of the STE20 family. Regulates Na-K-Cl cotransporter (NKCC) activity. Regulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton. Binds to and phosphorylates PAK1. Interacts and is phosphorylated by WNK1. Knockdown of either WNK1 or OXSR1 reduces NKCC activity. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress. An important paralog of this gene is STRADB.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; FRAY subfamily
Chromosomal Location of Human Ortholog: 3p22.2
Cellular Component: cytoplasm
Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: peptidyl-threonine phosphorylation; response to oxidative stress; protein amino acid phosphorylation
Research Articles on OXSR1
2. The WNK1-SPAK/OSR1 signalling pathway plays a key role in controlling the phosphorylation and activity of NCC.
3. The first crystal structure of an OSR1 fragment encompassing the catalytic domain of the enzyme, is reported.
4. These results provide the first molecular insight into the mechanism by which the SPAK and OSR1 kinases specifically recognize their upstream activators and downstream substrates.
5. OXSR1 kinase is shown to interact with the three RELT family members RELT, RELL1, and RELL2 by in vitro co-immunoprecipitation; additionally, OXSR1 phosphorylates RELT family members in an in vitro kinase assay.
6. isolation and characterization of OSR1 (oxidative stress-responsive 1), one of two human Fray homologs. OSR1 is a 58-kDa protein of 527 amino acids that is widely expressed in mammalian tissues and cell lines
7. WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling pathway to cation-chloride-coupled cotransporters
8. data establish that the CCT domain functions as a multipurpose docking site, enabling SPAK/OSR1 to interact with substrates (NKCC1) and activators (WNK1/WNK4)
9. OSR1 and sterile20-related, proline-, alanine-rich kinase are likely links between WNK lysine deficient protein kinase 1 and solute carrier family 12 in a pathway that contributes to volume regulation and blood pressure homeostasis in mammals
10. interacts with cation chloride cotransporters
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Product Notes
The OXSR1 oxsr1 (Catalog #AAA616581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OSR1 (Oxidative Stress Responsive 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OSR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:100 Immunofluorescence (IC): 1:25 Optimal dilutions to be determined by the researcher. Other applications not tested. Researchers should empirically determine the suitability of the OXSR1 oxsr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSR1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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