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Western Blot (WB) (Western Blot analysis of extracts from various cell types using MBS612850.)

Rabbit eEF1A Polyclonal Antibody | anti-EEF1A1 antibody

eEF1A (Eukaryotic Translation Elongation Factor 1 alpha 1)

Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; FLJ25721; GRAF-1EF; MGC16224; MGC102687; MGC131894; HNGC:16303
Reactivity
Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
eEF1A; Polyclonal Antibody; eEF1A (Eukaryotic Translation Elongation Factor 1 alpha 1); Anti -eEF1A (Eukaryotic Translation Elongation Factor 1 alpha 1); anti-EEF1A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total human eEF1A protein. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-EEF1A1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry.
Dilution: Immunofluorescence (IF-IC): 1:25
Western Blot: 1:1000; Band at ~50kD.
Immunogen
Synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Western Blot (WB)

(Western Blot analysis of extracts from various cell types using MBS612850.)

Western Blot (WB) (Western Blot analysis of extracts from various cell types using MBS612850.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS612850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of HeLa cells using MBS612850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Related Product Information for anti-EEF1A1 antibody
Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A variety of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells. eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and then interacts with amino acyl-tRNAs and promotes the recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level.
Product Categories/Family for anti-EEF1A1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,141 Da
NCBI Official Full Name
elongation factor 1-alpha 1
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol
EEF1A1
NCBI Official Synonym Symbols
CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; FLJ25721; GRAF-1EF; MGC16224; MGC102687; MGC131894; HNGC:16303
NCBI Protein Information
elongation factor 1-alpha 1; EF-1-alpha-1; EF1a-like protein; CTCL tumor antigen; OTTHUMP00000016736; OTTHUMP00000063804; OTTHUMP00000063805; elongation factor Tu; cervical cancer suppressor 3; elongation factor 1 alpha subunit; prostate tumor-inducing protein 1; eukaryotic elongation factor 1 A-1; leukocyte receptor cluster member 7; leukocyte receptor cluster (LRC) member 7; translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; eukaryotic translation elongation factor 1 alpha 1-like 14
UniProt Protein Name
Elongation factor 1-alpha 1
UniProt Gene Name
EEF1A1
UniProt Synonym Gene Names
EEF1A; EF1A; LENG7
UniProt Entry Name
EF1A1_HUMAN

NCBI Description

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq]

Uniprot Description

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protein type: Translation elongation; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: extracellular space; cortical actin cytoskeleton; eukaryotic translation elongation factor 1 complex; membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity; tRNA binding

Biological Process: cellular protein metabolic process; translational elongation; transcription, DNA-dependent; translation; regulation of transcription, DNA-dependent; gene expression

Research Articles on EEF1A1

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Product Notes

The EEF1A1 eef1a1 (Catalog #AAA612850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eEF1A (Eukaryotic Translation Elongation Factor 1 alpha 1) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eEF1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Dilution: Immunofluorescence (IF-IC): 1:25 Western Blot: 1:1000; Band at ~50kD. Researchers should empirically determine the suitability of the EEF1A1 eef1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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