Rabbit Actinin, alpha 1 Polyclonal Antibody | anti-ACTN1 antibody

Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle alpha-Actinin-1)

Cat. Num. AAA623096

• Gene Names: ACTN1; FLJ40884; FLJ54432
• Reactivity: Hamster, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Actinin; alpha 1; Polyclonal Antibody; alpha 1 (Alpha Actinin 1; alpha-Actinin-1; ACTN1; alpha-Actinin Cytoskeletal Isoform; F-actin Cross-linking Protein; FLJ40884; FLJ54432; Non-muscle alpha-Actinin-1); Anti -Actinin; anti-ACTN1 antibody

• Host: Rabbit
• Reactivity: Hamster, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Reognizes endogenous levels of total human alpha-actinin-1 protein. Based on sequence homology, the antibody may cross-react with alpha-actinin isoforms 2 and 4. Species crossreactivity: mouse, rat, hamster and monkey.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-ACTN1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence (IC): 1:25; Western Blot: 1:1000
• Immunogen: Synthetic peptide corresponding to carboxy-terminal residues of human alpha-actinin-1, conjugated to KLH (P12814).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from NIH/3T3, C6, CHO and COS cells using MBS623096.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NIH/3T3 cells using MBS623096 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-ACTN1 antibody

a-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, a-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of a-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of a-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of a-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).

Product Categories/Family for anti-ACTN1 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 30583253
• NCBI GeneID: 87
• UniProt Accession #: P12814; Q9BTN1; B3V8S3; B4DHH3; Q1HE25; Q5ZEZ4; Q86TX4
• Molecular Weight: 103,058 Da
• NCBI Official Full Name: actinin, alpha 1
• NCBI Official Synonym Full Names: actinin, alpha 1
• NCBI Official Symbol: ACTN1
• NCBI Official Synonym Symbols: FLJ40884; FLJ54432
• NCBI Protein Information: alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform
• UniProt Protein Name: Alpha-actinin-1
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN1
• UniProt Entry Name: ACTN1_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q24|14q22-q24

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; dendritic spine; extracellular region; pseudopodium; fascia adherens; Z disc; cytosol; ruffle; cell projection; cytoplasm; stress fiber; plasma membrane; nucleus

Molecular Function: integrin binding; actin filament binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding

Biological Process: regulation of apoptosis; focal adhesion formation; actin crosslink formation; platelet activation; actin filament bundle formation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation

Disease: Bleeding Disorder, Platelet-type, 15