Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS618246.)
Rabbit Eukaryotic Translation Initiation Factor 4H Polyclonal Antibody | anti-Eif4h antibody
Eukaryotic Translation Initiation Factor 4H (eIF4H)
Gene Names
Eif4h; Wbscr1; MGC94887
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Purified by protein A and peptide affinity chromatography.
Synonyms
Eukaryotic Translation Initiation Factor 4H; Polyclonal Antibody; Eukaryotic Translation Initiation Factor 4H (eIF4H); Anti -Eukaryotic Translation Initiation Factor 4H (eIF4H); anti-Eif4h antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of total eIF4H protein (both long and short isoforms).
Purity/Purification
Purified by protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Applicable Applications for anti-Eif4h antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Western blotting: 1:1000
Immunofluorescence: 1:100
Dilution: Western blotting: 1:1000
Immunofluorescence: 1:100
Immunogen
Synthetic peptide (KLH-coupled) derived from a sequence of human eIF4H.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS618246.)
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS618246.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PANC-1 cells using eIF4H Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PANC-1 cells using eIF4H Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Related Product Information for anti-Eif4h antibody
A variety of factors contribute to the initiation of translation. Eukaryotic initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3).
Product Categories/Family for anti-Eif4h antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,324 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4H
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4H
NCBI Official Symbol
Eif4h
NCBI Official Synonym Symbols
Wbscr1; MGC94887
NCBI Protein Information
eukaryotic translation initiation factor 4H; eIF-4H; Williams-Beuren syndrome chromosome region 1 homolog; williams-Beuren syndrome chromosomal region 1 protein homolog
UniProt Protein Name
Eukaryotic translation initiation factor 4H
Protein Family
UniProt Gene Name
Eif4h
UniProt Synonym Gene Names
Wbscr1
UniProt Entry Name
IF4H_RAT
Uniprot Description
EIF4H: eukaryotic translation initiation factor 4H stimulates protein translation. May stimulate the activities of EIF4A, EIF4B and EIF4F during the steps involving mRNA binding and utilization in the initiation of protein biosynthesis. Binds mRNA. Two alternatively spliced isoforms have been described. The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
Protein type: RNA-binding; Translation initiation; Translation
Cellular Component: membrane; perinuclear region of cytoplasm
Molecular Function: translation initiation factor activity; nucleotide binding
Biological Process: developmental growth; sexual reproduction; translational initiation
Similar Products
Product Notes
The Eif4h eif4h (Catalog #AAA618246) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eukaryotic Translation Initiation Factor 4H (eIF4H) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Eukaryotic Translation Initiation Factor 4H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Western blotting: 1:1000 Immunofluorescence: 1:100. Researchers should empirically determine the suitability of the Eif4h eif4h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eukaryotic Translation Initiation Factor 4H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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