Western Blot (WB)
(Western Blot analysis of extracts from THP-1, MDA-MB-468 and CAD cells, using MBS611815.)
Rabbit PI 4-Kinase Polyclonal Antibody | anti-PI4KA antibody
PI 4-Kinase (Phosphatidylinositol 4-Kinase)
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:100
Western Blot (WB)
(Western Blot analysis of extracts from THP-1, MDA-MB-468 and CAD cells, using MBS611815.)
Western Blot (WB)
(Western Blot analysis of extracts from THP-1, MDA-MB-468 and CAD cells, using MBS611815.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of C2C12 cells using MBS611815 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of C2C12 cells using MBS611815 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PIK4CA: Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes. This is a type II PtdIns-4-kinase activated by detergents such as triton and inhibited by adenosine. Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, lipid; EC 2.7.1.67; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: focal adhesion; membrane; plasma membrane; cytosol
Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding; ATP binding
Biological Process: phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; signal transduction
Disease: Polymicrogyria, Perisylvian, With Cerebellar Hypoplasia And Arthrogryposis
Research Articles on PI4KA
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Product Notes
The PI4KA pi4ka (Catalog #AAA611815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI 4-Kinase (Phosphatidylinositol 4-Kinase) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PI 4-Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:100. Researchers should empirically determine the suitability of the PI4KA pi4ka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI 4-Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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