CDKN1B recombinant protein

Recombinant CDKN1B protein

Cat. Num. AAA389434

• Gene Names: CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• Synonyms: CDKN1B; Recombinant CDKN1B protein; cyclin-dependent kinase inhibitor p27 orp27Kip1; Important regulator of cell cycle progression; CDKN1B recombinant protein

• Host: E Coli
• Form/Format: Recombinant CDKN1B protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 10% glycerol, 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Protein Details: Recombinant CDKN1B protein was expressed in E Coli as the full length protein (accession number NP_004055.1) with an N-terminal 6×His-tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant CDKN1B protein 12.5% SDS-PAGE Coomassie staining M.W.: 24.01 kDa Purity: ? 80%)

Related Product Information for CDKN1B recombinant protein

Background: CDKN1B is a cyclin-dependent kinase inhibitor,also called cyclin-dependent kinase inhibitor p27 or p27Kip1,Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA.Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Product Categories/Family for CDKN1B recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4757962
• NCBI GeneID: 1027
• NCBI Accession #: NP_004055.1
• NCBI GenBank Nucleotide #: NM_004064.3
• UniProt Accession #: P46527; Q16307; Q5U0H2; Q9BUS6
• Molecular Weight: 22,073 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1B
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• NCBI Official Symbol: CDKN1B
• NCBI Official Synonym Symbols: KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1B
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1B
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: CDKN1B
• UniProt Synonym Gene Names: KIP1
• UniProt Entry Name: CDN1B_HUMAN

NCBI Description

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq, Jul 2008]

Uniprot Description

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Protein type: Oncoprotein; Cell cycle regulation; Inhibitor; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12p13.1-p12

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus; endosome

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; caspase activator activity; Hsp70 protein binding; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: response to peptide hormone stimulus; negative regulation of kinase activity; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; response to drug; caspase activation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; autophagic cell death; positive regulation of protein catabolic process; response to hypoxia; negative regulation of phosphorylation; innate immune response; negative regulation of cell growth; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

Disease: Multiple Endocrine Neoplasia, Type Iv