Rabbit FRG1 Polyclonal Antibody | anti-FRG1 antibody

Anti-FRG1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102218

• Reactivity: Mouse; Predicted to React with: Human (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: FRG1; Polyclonal Antibody; Anti-FRG1 Antibody; Rabbit Polyclonal; FRG1 Antibody; Rabbit PAb; Antigen Affinity Purified; FSHD region gene 1; anti-FRG1 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse FRG1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FRG1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Mouse FRG1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse FRG1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Frg1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit  IgG (H&L) /Dylight 800  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of Frg1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human Frg1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Immunoprecipitation (IP)

(Frg1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg A549 Whole Cell Lysate 2 uL anti-Frg1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Frg1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 38 kDa)

Related Product Information for anti-FRG1 antibody

Facioscapulohumeral muscular dystrophy (FSHD) is a common muscle disease. Over-expression of FSHD region gene 1 (FRG1) in mice, frogs, and worms perturbs muscle development and causes FSHD-like phenotypes. FRG1 is also important for the normal muscular and vascular development. FRG1 is involved in the morphogenesis of the tooth germ, as well as in the formation of enamel and dentin matrices and that FRG1 may play a role in the odontogenesis in the mouse following BMP4 stimulation. In mammalian cells, FRG1 is a dynamic nuclear and cytoplasmic protein, however in muscle, FRG1 is also a developmentally regulated sarcomeric protein suggesting FRG1 may perform a muscle-specific function. Thus, FRG1 is the only FSHD candidate protein linked to the muscle contractile machinery and may address why the musculature and vasculature are specifically susceptible in FSHD. The FRG1 protein is highly conserved and contains a lipocalin sequence motif, suggesting it may function as a transport protein.

NCBI and Uniprot Product Information

• NCBI GI #: 225543552
• NCBI GeneID: 14300
• NCBI Accession #: NP_038550.2
• NCBI GenBank Nucleotide #: NM_013522.3
• UniProt Accession #: P97376; Q99M42
• Molecular Weight: 29,127 Da
• NCBI Official Full Name: protein FRG1
• NCBI Official Synonym Full Names: FSHD region gene 1
• NCBI Official Symbol: Frg1
• NCBI Protein Information: protein FRG1; FSHD region gene 1 protein
• UniProt Protein Name: Protein FRG1
• Protein Family: Protein
• UniProt Gene Name: Frg1
• UniProt Entry Name: FRG1_MOUSE