Ctsl recombinant protein

Ctsl, 18-334aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206250

• Gene Names: Ctsl; fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Ctsl; 18-334aa; Mouse; His tag; Baculovirus; Cathepsin L1; CTSL; 1190035F06Rik; CatL; Ctsl1; fs; MEP; nkt; Ctsl recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: TPKFDQTFSA EWHQWKSTHR RLYGTNEEEW RRAIWEKNMR MIQLHNGEYS NGQHGFSMEM NAFGDMTNEE FRQVVNGYRH QKHKKGRLFQ EPLMLKIPKS VDWREKGCVT PVKNQGQCGS CWAFSASGCL EGQMFLKTGK LISLSEQNLV DCSHAQGNQG CNGGLMDFAF QYIKENGGLD SEESYPYEAK DGSCKYRAEF AVANDTGFVD IPQQEKALMK AVATVGPISV AMDASHPSLQ FYSSGIYYEP NCSSKNLDHG VLLVGYGYEG TDSNKNKYWL VKNSWGSEWG MEGYIKIAKD RDNHCGLATA ASYPVVNLEH HHHHH
• Sequence Length: 334

• Applicable Applications for Ctsl recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ctsl recombinant protein

CTSL, also known as cathepsin L1, is a lysosomal cysteine protease expressed in most eukaryotic cells. It plays a major role in intracellular protein catabolism and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. Recombinant mouse CTSL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ctsl recombinant protein

Neuroscience; Adhesion & Movement

References

Goretzki L., et al. (1992) FEBS Lett. 297:112-118.; Taggart CC., et al. (2001) J Biol Chem. 276:33345-33352.

NCBI and Uniprot Product Information

• NCBI GI #: 6753558
• NCBI GeneID: 13039
• NCBI Accession #: NP_034114
• NCBI GenBank Nucleotide #: NM_009984.4
• UniProt Accession #: P06797; Q91UZ0
• Molecular Weight: 36.8kDa (325aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: cathepsin L1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: Ctsl
• NCBI Official Synonym Symbols: fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• NCBI Protein Information: cathepsin L1
• UniProt Protein Name: Cathepsin L1
• UniProt Gene Name: Ctsl
• UniProt Synonym Gene Names: Ctsl1; MEP

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

Important for the overall degradation of proteins in lysosomes.