Rabbit anti-Human MVP Polyclonal Antibody | anti-MVP antibody

Anti-MVP Antibody, Rabbit Polyclonal

Cat. Num. AAA8101783

• Gene Names: MVP; LRP; VAULT1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MVP; Polyclonal Antibody; Anti-MVP Antibody; Rabbit Polyclonal; MVP/major vault protein Antibody; Rabbit PAb; Antigen Affinity Purified; major vault protein; Anti-LRP Antibody; Anti-VAULT1 Antibody; anti-MVP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MVP/major vault protein
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MVP antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human MVP/major vault protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MVP/major vault protein, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MVP rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:110 kDaObserved band size:120 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of MVP in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MVP polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Immunoprecipitation (IP)

(MVP was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-MVP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MVP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)

Related Product Information for anti-MVP antibody

MVP (Major Vault Protein) is a Protein Coding gene. This gene encodes the major component of the vault complex. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT, and phosphoinositide 3-kinase/Akt signaling pathways. MVP is a ribonucleoprotein comprising a major part (>70%) of the vault particle. The human MVP is also known as lung resistance-related protein (LRP) represents the predominant component of vaults that may be involved in the defense against xenobiotics. The human MVP has been implicated in the development of drug resistance in cancer cells. MVP has been implicated in multidrug resistance, cellular transport, and malignant transformation. Overexpression of MVP has also been reported in brain tissue samples from the antiepileptic drug (AED)-resistant human focal epilepsies.

NCBI and Uniprot Product Information

• NCBI GI #: 19913412
• NCBI GeneID: 9961
• NCBI Accession #: NP_005106.2
• NCBI GenBank Nucleotide #: NM_005115.4
• UniProt Accession #: Q14764; Q96BG4; Q9BPW6; Q9BQT1; Q9UBD1
• Molecular Weight: 99,327 Da
• NCBI Official Full Name: major vault protein
• NCBI Official Synonym Full Names: major vault protein
• NCBI Official Symbol: MVP
• NCBI Official Synonym Symbols: LRP; VAULT1
• NCBI Protein Information: major vault protein; lung resistance-related protein
• UniProt Protein Name: Major vault protein
• Protein Family: Hyperpolarization-activated voltage-gated potassium channel
• UniProt Gene Name: MVP
• UniProt Synonym Gene Names: LRP; MVP
• UniProt Entry Name: MVP_HUMAN

NCBI Description

This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

MVP: Required for normal vault structure. Vaults are multi- subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo- cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoskeleton; membrane; perinuclear region of cytoplasm; cytoplasm; nuclear pore; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; protein phosphatase binding; protein kinase binding

Biological Process: cell proliferation; protein transport; mRNA transport; negative regulation of protein amino acid autophosphorylation