Rabbit anti-Human Citrate Synthase Polyclonal Antibody | anti-CS antibody

Anti-Citrate Synthase Antibody, Rabbit Polyclonal

Cat. Num. AAA8107230

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Citrate Synthase; Polyclonal Antibody; Anti-Citrate Synthase Antibody; Rabbit Polyclonal; citrate synthase/CS Antibody; Rabbit PAb; Antigen Affinity Purified; citrate synthase; anti-CS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human citrate synthase/CS
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CS antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human citrate synthase/CS Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human citrate synthase/CS (rh citrate synthase/CS). citrate synthase/CS specific IgG was purified by Human citrate synthase/CS affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CS rabbit polyclonal antibody at 1:500 dilution Lane A: A375 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Caco-2 Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:51 kDa Observed band size:45 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human CS in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CS in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of CS in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CS polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Immunoprecipitation (IP)

(CS was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate Lane C:0.5 mg Caco-2 Whole Cell Lysate 4 uL anti-CS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa)

Related Product Information for anti-CS antibody

Chondroitin sulphate (CS) glycosaminoglycan chains on cell and extracellular matrix proteoglycans (PGs) can no longer be regarded as merely hydrodynamic space fillers. Overwhelming evidence over recent years indicates that sulphation motif sequences within the CS chain structure are a source of significant biological information to cells and their surrounding environment. CS sulphation motifs have been shown to interact with a wide variety of bioactive molecules, e.g. cytokines, growth factors, chemokines, morphogenetic proteins, enzymes and enzyme inhibitors, as well as structural components within the extracellular milieu. They are therefore capable of modulating a panoply of signalling pathways, thus controlling diverse cellular behaviours including proliferation, differentiation, migration and matrix synthesis. Chondroitin sulfate (CS) is a sulfated glycosaminoglycan composed of a long chain of repeating disaccharide units that are attached to core proteins, resulting in CS proteoglycans (CSPGs). In the mature brain, CS is concentrated in perineuronal nets (PNNs), which are extracellular structures that surround synapses and regulate synaptic plasticity. In addition, CS is rapidly synthesized after CNS injury to create a physical and chemical barrier that inhibits axon growth.

NCBI and Uniprot Product Information

• NCBI GI #: 38327625
• NCBI GeneID: 1431
• NCBI Accession #: NP_004068.2
• NCBI GenBank Nucleotide #: NM_004077.2
• UniProt Accession #: O75390; Q71UT9; Q7KZH0; Q96FZ8; Q9BWN8
• Molecular Weight: 51,712 Da
• NCBI Official Full Name: citrate synthase, mitochondrial
• NCBI Official Synonym Full Names: citrate synthase
• NCBI Official Symbol: CS
• NCBI Protein Information: citrate synthase, mitochondrial
• UniProt Protein Name: Citrate synthase, mitochondrial
• Protein Family: Citrate synthase
• UniProt Gene Name: CS
• UniProt Entry Name: CISY_HUMAN

Uniprot Description

CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 2.3.3.1; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Molecular Function: citrate (Si)-synthase activity

Biological Process: carbohydrate metabolic process; tricarboxylic acid cycle