Rabbit Mineralocorticoid Receptor/NR3C2 Polyclonal Antibody | anti-NR3C2 antibody

Anti-Mineralocorticoid Receptor/NR3C2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101450

• Gene Names: NR3C2; MR; MCR; MLR; NR3C2VIT
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Mineralocorticoid Receptor/NR3C2; Polyclonal Antibody; Anti-Mineralocorticoid Receptor/NR3C2 Antibody; Rabbit Polyclonal; NR3C2 Antibody; Rabbit PAb; Antigen Affinity Purified; nuclear receptor subfamily 3; group C; member 2; Anti-MCR Antibody; Anti-MLR Antibody; Anti-MR Antibody; Anti-NR3C2VIT Antibody; anti-NR3C2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Mineralocorticoid Receptor/NR3C2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NR3C2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human NR3C2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NR3C2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NR3C2 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of NR3C2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NR3C2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(NR3C2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-NR3C2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NR3C2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 107 kDaObserved band size: 107 kDa)

Related Product Information for anti-NR3C2 antibody

NR3C2 (Nuclear Receptor Subfamily 3 Group C Member 2, also known as MR and MCR) is a Protein Coding gene. This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. It belongs to the nuclear hormone receptor family. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements to transactivate target genes. The mineralocorticoid receptor (NR3C2) plays important role in the modulation of blood pressure. NR3C2 has previously been described as a tumor suppressor gene in several cancers. Diseases associated with NR3C2 include Pseudohypoaldosteronism, Type I, Autosomal Dominant and Hypertension, Early-Onset, Autosomal Dominant, With Severe Exacerbation In Pregnancy.

NCBI and Uniprot Product Information

• NCBI GI #: 126885
• NCBI GeneID: 4306
• UniProt Accession #: P08235; Q2NKL1; Q96KQ8; Q96KQ9; B0ZBF5; B0ZBF7
• Molecular Weight: 984
• NCBI Official Full Name: Mineralocorticoid receptor
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3, group C, member 2
• NCBI Official Symbol: NR3C2
• NCBI Official Synonym Symbols: MR; MCR; MLR; NR3C2VIT
• NCBI Protein Information: mineralocorticoid receptor; aldosterone receptor; mineralocorticoid receptor 1; mineralocorticoid receptor delta
• UniProt Protein Name: Mineralocorticoid receptor
• Protein Family: Mineralocorticoid receptor
• UniProt Gene Name: NR3C2
• UniProt Synonym Gene Names: MCR; MLR; MR
• UniProt Entry Name: MCR_HUMAN

NCBI Description

This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

MCR: Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1). PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported. Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP). Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. Belongs to the nuclear hormone receptor family. NR3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 4q31.1

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; receptor complex

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; signal transduction

Disease: Hypertension, Early-onset, Autosomal Dominant, With Severe Exacerbation In Pregnancy; Pseudohypoaldosteronism, Type I, Autosomal Dominant