Rabbit anti-Human GALK1 Polyclonal Antibody | anti-GALK1 antibody

Anti-GALK1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105292

• Gene Names: GALK1; GK1; GALK
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: GALK1; Polyclonal Antibody; Anti-GALK1 Antibody; Rabbit Polyclonal; GALK1 Antibody; Rabbit PAb; Antigen Affinity Purified; galactokinase 1; Anti-GALK Antibody; Anti-GK1 Antibody; Anti-HEL-S-19 Antibody; anti-GALK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GALK1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GALK1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human GALK1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human GALK1 (rh GALK1). GALK1 specific IgG was purified by Human GALK1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa (We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- HG3C-GALK1 polyclonal antibody (dilution ratio: 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.)

Immunoprecipitation (IP)

(GALK1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-GALK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-GALK1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)

Western Blot (WB)

(Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution Lane A: GALK1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa)

Related Product Information for anti-GALK1 antibody

Galactokinase, also known as Galactose kinase, GALK and GALK1, is a protein which belongs to theGHMP kinase family and GalK subfamily. Galactokinase/GALK1 is a major enzyme for galactose metabolism. Galactokinase (GALK) deficiency is an autosomal recessive disorder characterized by elevation of blood galactose concentration and diminished galactose-1-phosphate, leading to the production of galactitol. Defects in GALK1 are the cause of galactosemia II (GALCT2) which II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses.

References

Hunter, M. et al., 2001, Hum Mutat. 17 (1):77-8. Park, H.D. et al., 2007, Mol Genet Metab. 91 (3):234-8. Park, H.D. et al., 2009, BMC Med Genet. 10 :29.

NCBI and Uniprot Product Information

• NCBI GI #: 4503895
• NCBI GeneID: 2584
• NCBI Accession #: NP_000145.1
• NCBI GenBank Nucleotide #: NM_000154.1
• UniProt Accession #: P51570; B2RC07
• Molecular Weight: 42,272 Da
• NCBI Official Full Name: galactokinase
• NCBI Official Synonym Full Names: galactokinase 1
• NCBI Official Symbol: GALK1
• NCBI Official Synonym Symbols: GK1; GALK
• NCBI Protein Information: galactokinase; galactose kinase
• UniProt Protein Name: Galactokinase
• UniProt Gene Name: GALK1
• UniProt Synonym Gene Names: GALK
• UniProt Entry Name: GALK1_HUMAN

NCBI Description

Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts during infancy and presenile cataracts in the adult population. [provided by RefSeq, Jul 2008]

Uniprot Description

GALK1: Major enzyme for galactose metabolism. Defects in GALK1 are the cause of galactosemia II (GALCT2). Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses. Belongs to the GHMP kinase family. GalK subfamily.

Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; EC 2.7.1.6

Chromosomal Location of Human Ortholog: 17q24

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: galactokinase activity; galactose binding; ATP binding

Biological Process: galactose catabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; galactitol metabolic process; galactose metabolic process; pathogenesis

Disease: Galactokinase Deficiency