Rabbit Nup107 Polyclonal Antibody | anti-NUP107 antibody

Anti-Nup107 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101770

• Gene Names: NUP107; NUP84
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Nup107; Polyclonal Antibody; Anti-Nup107 Antibody; Rabbit Polyclonal; NUP107 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-NUP84 Antibody; anti-NUP107 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Nup107
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NUP107 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human NUP107
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NUP107, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NUP107 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:106 kDaObserved band size:115 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of NUP107 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- NUP107 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Immunoprecipitation (IP)

(NUP107 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate1 uL anti-NUP107 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NUP107 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 106 kDaObserved band size: 106 kDa)

Related Product Information for anti-NUP107 antibody

Nucleoporin 107 (NUP107), a protein involved in transport between cytoplasm and nucleus with putative roles in meiosis/mitosis progression, was recently implicated as a cause of HH. NUP107 is a novel gene associated with autosomal recessive steroid-resistant nephrotic syndrome (SRNS) with focal segmental glomerulosclerosis (FSGS) in children. Nucleoporin 107 kDa (NUP107) is a component of the NPC central scaffold and is an essential protein in all eukaryotic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 9966881
• NCBI GeneID: 57122
• NCBI Accession #: NP_065134.1
• NCBI GenBank Nucleotide #: NM_020401.2
• UniProt Accession #: P57740
• Molecular Weight: 106,374 Da
• NCBI Official Full Name: nuclear pore complex protein Nup107
• NCBI Official Synonym Full Names: nucleoporin 107kDa
• NCBI Official Symbol: NUP107
• NCBI Official Synonym Symbols: NUP84
• NCBI Protein Information: nuclear pore complex protein Nup107; nucleoporin Nup107; 107 kDa nucleoporin
• UniProt Protein Name: Nuclear pore complex protein Nup107
• Protein Family: Nucleoporin
• UniProt Gene Name: NUP107
• UniProt Entry Name: NU107_HUMAN

NCBI Description

This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NUP107: Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex. Belongs to the nucleoporin Nup84/Nup107 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nucleoplasm; kinetochore; centrosome; nuclear membrane; membrane; nuclear pore; nuclear envelope; cytosol

Molecular Function: protein binding; structural constituent of nuclear pore; nucleocytoplasmic transporter activity

Biological Process: nuclear pore complex assembly; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; mRNA export from nucleus; protein transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport