Immunofluorescence (IF)
(Immunofluorescence staining of ASF1A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ASF1A polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Rabbit ASF1A Polyclonal Antibody | anti-ASF1A antibody
Anti-ASF1A Antibody, Rabbit Polyclonal
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
ICC/IF: 1:300-1:10,000
IP: 1-4uL/mg of lysate
Immunofluorescence (IF)
(Immunofluorescence staining of ASF1A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ASF1A polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of ASF1A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ASF1A polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Immunoprecipitation (IP)
(ASF1A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-ASF1A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ASF1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 35 kDa)
Immunoprecipitation (IP)
(ASF1A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-ASF1A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ASF1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 35 kDa)
Western Blot (WB)
(Anti-ASF1A rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ASF1A rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]
Uniprot Description
ASF1A: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Ubiquitously expressed. Belongs to the ASF1 family.
Protein type: DNA repair, damage; Histone-binding
Chromosomal Location of Human Ortholog: 6q22.31
Cellular Component: nucleoplasm; protein complex; nuclear chromatin; nucleus
Molecular Function: protein binding; histone binding; chromatin binding
Biological Process: osteoblast differentiation; nucleosome assembly; muscle cell differentiation; transcription, DNA-dependent; negative regulation of chromatin silencing; DNA replication-independent nucleosome assembly; chromatin modification; DNA repair; DNA replication-dependent nucleosome assembly
Research Articles on ASF1A
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Product Notes
The ASF1A asf1a (Catalog #AAA8100724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ASF1A Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's ASF1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ICC/IF: 1:300-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the ASF1A asf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASF1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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