Rabbit anti-Human FEN1 Polyclonal Antibody | anti-FEN1 antibody

Anti-FEN1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100793

• Gene Names: FEN1; MF1; RAD2; FEN-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: FEN1; Polyclonal Antibody; Anti-FEN1 Antibody; Rabbit Polyclonal; FEN1 Antibody; Rabbit PAb; Antigen Affinity Purified; flap structure-specific endonuclease 1; Anti-FEN-1 Antibody; Anti-MF1 Antibody; Anti-RAD2 Antibody; anti-FEN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human FEN1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FEN1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:1000-1:4000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human FEN1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human FEN1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of FEN1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FEN1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)

Immunohistochemistry (IHC)

(Immunochemical staining of human FEN1 in human tonsil with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human FEN1 in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(FEN1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-FEN1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FEN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)

Western Blot (WB)

(Anti-FEN1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:48 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-FEN1 antibody

The structure-specific nuclease human flap endonuclease-1 (hFEN1) plays a key role in DNA replication and repair and may be of interest as an oncology target. FEN1 phosphorylation is proposed to regulate the action of FEN1 in DNA repair as well as Okazaki fragment maturation. FEN1 polymorphisms may reduce the risk of breast cancer in Chinese women. FEN1(Flap endonuclease 1) is a central component in cellular metabolism, overexpression and decrease of FEN1 levels may cause cancer, these regulation changes of Flap endonuclease 1reported in many cancer cells. Suppression of FEN1 resulted in decreased DNA replication and accumulation of DNA damage, which subsequently induced apoptosis. Manipulating the amount of FEN1 altered the response of lung cancer cells to chemotherapeutic drugs. A small-molecule inhibitor (C20) was used to target FEN1 and this enhanced the therapeutic effect of cisplatin. The FEN1 inhibitor significantly suppressed cell proliferation and induced DNA damage in lung cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4758356
• NCBI GeneID: 2237
• NCBI Accession #: NP_004102.1
• NCBI GenBank Nucleotide #: NM_004111.5
• UniProt Accession #: P39748; AAB32522
• Molecular Weight: 42,593 Da
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure-specific endonuclease 1
• NCBI Official Symbol: FEN1
• NCBI Official Synonym Symbols: MF1; RAD2; FEN-1
• NCBI Protein Information: flap endonuclease 1; DNase IV; maturation factor 1; maturation factor-1
• UniProt Protein Name: Flap endonuclease 1
• UniProt Gene Name: FEN1
• UniProt Synonym Gene Names: RAD2; FEN-1; MF1; hFEN-1
• UniProt Entry Name: FEN1_HUMAN

NCBI Description

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Uniprot Description

FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein type: Ribonuclease; DNA repair, damage; Nucleolus; EC 3.1.-.-; Nuclear receptor co-regulator; DNA-binding; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; membrane; mitochondrion; plasma membrane; nucleolus; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding; DNA binding; 5'-flap endonuclease activity; manganese ion binding; endonuclease activity; exonuclease activity; double-stranded DNA binding; damaged DNA binding; magnesium ion binding; ribonuclease H activity; double-stranded DNA specific exodeoxyribonuclease activity

Biological Process: telomere maintenance via semi-conservative replication; DNA replication, removal of RNA primer; UV protection; base-excision repair; double-strand break repair; telomere maintenance via recombination; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA catabolic process, exonucleolytic; DNA repair; telomere maintenance; DNA catabolic process, endonucleolytic; memory